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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [3]
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Validation data
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- Product number
- TA502826 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502826, RRID:AB_11129803
- Product name
- PNMT mouse monoclonal antibody, clone OTI2D5 (formerly 2D5)
- Antibody type
- Monoclonal
- Description
- PNMT mouse monoclonal antibody, clone OTI2D5 (formerly 2D5)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- PNMT
- Isotype
- IgG
- Antibody clone number
- OTI2D5
- Vial size
- 100 µl
- Concentration
- 0.7 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PNMT (RC206586, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PNMT.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-PNMT mouse monoclonal antibody (TA502826).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-PNMT mouse monoclonal antibody (TA502826) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PNMT(RC206586 ).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502826)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-PNMT antibody(TA502826),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC206586 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PNMT antibody(TA502826), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-PNMT antibody(TA502826),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
