MA5-25530

antibody from Invitrogen Antibodies

Targeting: PNMT PENT

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-25530

Provider

Invitrogen Antibodies

Product name

PNMT Monoclonal Antibody (OTI1D2)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Mouse, Rat, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

OTI1D2

Vial size

100 µL

Concentration

1.0 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

β-Adrenoceptor Activation in Breast MCF-10A Cells Induces a Pattern of Catecholamine Production Similar to that of Tumorigenic MCF-7 Cells.

Amaro F, Silva D, Reguengo H, Oliveira JC, Quintas C, Vale N, Gonçalves J, Fresco P
International journal of molecular sciences 2020 Oct 27;21(21)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PNMT in HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12, MCF7 cells using 35 µg per lane. Samples were probed with PNMT (Product # MA5-25530) monoclonal antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PNMT in human tissue (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon) samples using 10 µg per lane. Samples were separated by SDS-PAGE and probed with PNMT (Product # MA5-25530) monoclonal antibody at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PNMT in HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12, MCF7 cells using 35 µg per lane. Samples were probed with PNMT (Product # MA5-25530) monoclonal antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PNMT in COS7 cells. Cells were transfected with a plasmid overexpressing PNMT and probed with a PNMT monoclonal antibody (Product # MA5-25530).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded human endometrium tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a PNMT monoclonal antibody (Product # MA5-25530).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded carcinoma of human bladder tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a PNMT monoclonal antibody (Product # MA5-25530).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human endometrium tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a PNMT monoclonal antibody (Product # MA5-25530).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 Protein expression of tyrosine hydroxylase (TH) and phenylethanolamine N-methyltransferase (PNMT) in MCF-10A or in MCF-7 cells, revealed by immunocytochemistry. Shown are representative microphotographs of immunoreactivities revealed by secondary antibodies conjugated with Alexa fluor 594 (red fluorescence, TH) or with Alexa fluor 488 (green fluorescence, PNMT). Nuclei were labelled with Hoechst 33342 (blue fluorescence). Scale bar: 20 um. Negative control (CTR): without primary antibody.