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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-29916 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PNMT Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2.
- Concentration
- 0.69 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PNMT Polyclonal Antibody (Product # PA5-29916). Sample (30 µg of whole cell lysate). Lane A: HepG2. 12% SDS PAGE. PNMT Polyclonal Antibody (Product # PA5-29916) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PNMT Polyclonal Antibody (Product # PA5-29916). Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with PNMT Polyclonal Antibody (Product # PA5-29916) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded U87 xenograft, using PNMT (Product # PA5-29916) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
