F54856

antibody from NSJ Bioreagents

Targeting: CNOT8 CAF1, CALIF, hCAF1, POP2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54856

Provider

NSJ Bioreagents

Product name

CNOT8 Antibody / CCR4-NOT transcription complex subunit 8

Antibody type

Polyclonal

Description

This highly specific CNOT8 antibody is suitable for use in Western blot/Immunofluorescence/Immunohistochemistry/Flow cytometry applications with human samples.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the CNOT8 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human U-251 cell lysate with CNOT8 antibody. Predicted molecular weight ~33 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of fixed and permeabilized human HeLa cells with CNOT8 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human colon carcinoma tissue with CNOT8 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human CCRF-CEM cells with CNOT8 antibody; Blue=isotype control, Green= CNOT8 antibody.