HPA049137

antibody from Atlas Antibodies

Targeting: PLOD1 LH1, LLH, PLOD

Western blot (Enhanced validation)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA049137

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA049137, RRID:AB_2680649

Product name

Anti-PLOD1

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

YISNIYLIKGSALRGELQSSDLFHHSKLDPDMAFC
ANIRQQDVFMFLTNRHTLGHLLSLDSYRTTHLHND
LWEVFSN


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell line U-251 MG and human cell line RT-4.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Western blot analysis using Anti-PLOD1 antibody HPA049137 (A) shows similar pattern to independent antibody HPA055799 (B).

Sample type

HUMAN

Antibody #2 product nr

HPA055799

Antibody provider

Atlas Antibodies

Independent Antibody Method

WB

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line U-251 MG.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human placenta shows cytoplasmic positivity in decidual cells.