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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- 710377 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TPL2 Recombinant Polyclonal Antibody (3HCLC)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 3HCLC
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Janus Kinase 1 Plays a Critical Role in Mammary Cancer Progression.
Wehde BL, Rädler PD, Shrestha H, Johnson SJ, Triplett AA, Wagner KU
Cell reports 2018 Nov 20;25(8):2192-2207.e5
Cell reports 2018 Nov 20;25(8):2192-2207.e5
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TPL2 in whole cell extracts from Jurkat cells (lane 1), 3T3L1 cells (lane 2), and HepG2 cells (lane 3) using a TPL2 Recombinant Rabbit Polyclonal Antibody (Product # 710377) at a dilution of 2 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~52kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of COT/TPL2/MAP3K8 was done on 70% confluent log phase U-87MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with COT/TPL2/MAP3K8 (3HCLC), Recombinant Rabbit Polyclonal Antibody (Product # 710377) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
