40-1000

antibody from Invitrogen Antibodies

Targeting: NACA NACA1

Western blot (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

40-1000

Provider

Invitrogen Antibodies

Product name

NACA Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

0.25 mg/mL

Storage

-20°C

References

Submitted references

Utility of Claudin-3 in extracellular vesicles from human bile as biomarkers of cholangiocarcinoma.

Ikeda C, Haga H, Makino N, Inuzuka T, Kurimoto A, Ueda T, Matsuda A, Kakizaki Y, Ishizawa T, Kobayashi T, Sugahara S, Tsunoda M, Suda K, Ueno Y
Scientific reports 2021 Jan 13;11(1):1195

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Comparison of the PBS and EDEG groups in EVs. ( A ) TEM image of EVs derived from human bile samples. Both the PBS and EDEG groups contained circular and cup-shaped nanoparticles, which are representative of EVs. The scale bar represents 100 nm. ( B ) Western blot analysis of exosome marker proteins (CD9, ALIX, and Flollitin-2). Exosome marker proteins were detected in both EDEG and PBS groups. ( C ) Nanoparticle tracking analysis of human bile-derived EVs. Representative particle size distribution maps of the PBS and EDEG groups are shown. The particle concentration was 1.92 x 10 12 /mL in the PBS group and 1.93 x 10 12 /mL in the EDEG group. The particle mode was 82.4 nm in the PBS group and 78.9 nm in the EDEG group and was smaller in the EDEG group. ( D ) Comparison of the concentration of RNA extracted from EVs as measured using Qubit 3.0 Fluorometer. The RNA concentration was 2.32 +- 0.96 ng/uL in the PBS group and 4.56 +- 1.03 ng/uL in the EDEG group, which indicated that RNA concentration was significantly higher in the EDEG group. *p < 0.05. ( E ) Bioanalyzer electropherograms of the size distribution of miRNA from EVs. The percentage of microRNA (10-40 nt) out of the small RNA (6-150 nt) detected was 84% in the PBS group and 97% in the EDEG group. The miRNA concentration was 1.96 ng/uL in the PBS group and 3.08 ng/uL in the EDEG group. Lower marker peak was at 4 nt, FU fluorescence units.