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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- NB600-920-100ug - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Ferritin Heavy Chain Antibody
- Antibody type
- Polyclonal
- Description
- Ion exchange chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ferritin [Human Spleen]. Cross-reactivity against Ferritin from other tissues and species may occur but have not been specifically determined.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Ferritin Antibody [NB600-920] - Lane 1: Ferritin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Ferritin primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 20 kDa, 20 kDa for Ferritin. Other band(s): None.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Ferritin Antibody [NB600-920] - Proteins extracts (1000ug) of HT1080 fibrosarcoma cells were subjected to immunoprecipitation using 5ul of NB600-920 anti-ferritin antibody.
