M02401

antibody from Boster Biological Technology

Targeting: FTH1 FHC, FTH, FTHL6, PIG15, PLIF

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

M02401

Provider

Boster Biological Technology

Product name

Anti-Ferritin FTH1 Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for FERRITIN HEAVY CHAIN/FTH1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IF, ICC, WB. Reactive species: Human, Mouse, Rat FERRITIN HEAVY CHAIN/FTH1 information: Molecular Weight: 21226 MW; Tissue Specificity: Expressed in the liver.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

FBA-6

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

References

Submitted references

Cinnamaldehyde alleviates aspirin-induced gastric mucosal injury by regulating pi3k/akt pathway-mediated apoptosis, autophagy and ferroptosis.

Yan S, Bao S, Chen T, Chen J, Zhang J, Hu X, Liang Y, Zhou X, Li J
Phytomedicine : international journal of phytotherapy and phytopharmacology 2024 Sep;132:155791

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Fluoride Induces Neurocytotoxicity by Disrupting Lysosomal Iron Metabolism and Membrane Permeability.

Xu P, Xing H, Ma Y, Ding X, Li T, Zhang Y, Liu L, Ma J, Niu Q
Biological trace element research 2024 May 18;

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New insights into the spleen injury by mitochondrial dysfunction of chicken under polystyrene microplastics stress.

Guo T, Geng X, Zhang Y, Hou L, Lu H, Xing M, Wang Y
Poultry science 2024 Jun;103(6):103674

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Characterization of PKCα-rutin interactions and their application as a treatment strategy for pulmonary arterial hypertension by inhibiting ferroptosis.

Che H, Yi J, Zhao X, Yu H, Wang X, Zhang R, Li X, Fu J, Li Q
Food & function 2024 Jan 22;15(2):779-793

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Activation of the Nrf-2 pathway by pinocembrin safeguards vertebral endplate chondrocytes against apoptosis and degeneration caused by oxidative stress.

Wang H, Liu X, Yang H, Jing X, Wang W, Liu X, Zhang B, Liu X, Shao Y, Cui X
Life sciences 2023 Nov 15;333:122162

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Astaxanthin suppresses oxidative stress and calcification in vertebral cartilage endplate via activating Nrf-2/HO-1 signaling pathway.

Yang G, Liu X, Jing X, Wang J, Wang H, Chen F, Wang W, Shao Y, Cui X
International immunopharmacology 2023 Jun;119:110159

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Cross-talk between autophagy and ferroptosis contributes to the liver injury induced by fluoride via the mtROS-dependent pathway.

Xu W, Hu Z, Zhang J, Tang Y, Xing H, Xu P, Ma Y, Niu Q
Ecotoxicology and environmental safety 2023 Jan 15;250:114490

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Transmissible Endoplasmic Reticulum Stress Mediated by Extracellular Vesicles from Adipocyte Promoting the Senescence of Adipose-Derived Mesenchymal Stem Cells in Hypertrophic Obesity.

Fang J, Li L, Cao X, Yue H, Fu W, Chen Y, Xu Z, Zhao Q, Zhao J, Wang Y, Liang W
Oxidative medicine and cellular longevity 2022;2022:7175027

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HO-1 Contributes to Luteolin-Triggered Ferroptosis in Clear Cell Renal Cell Carcinoma via Increasing the Labile Iron Pool and Promoting Lipid Peroxidation.

Han S, Lin F, Qi Y, Liu C, Zhou L, Xia Y, Chen K, Xing J, Liu Z, Yu W, Zhang Y, Zhou X, Rao T, Cheng F
Oxidative medicine and cellular longevity 2022;2022:3846217

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Icariin protects vertebral endplate chondrocytes against apoptosis and degeneration via activating Nrf-2/HO-1 pathway.

Shao Y, Sun L, Yang G, Wang W, Liu X, Du T, Chen F, Jing X, Cui X
Frontiers in pharmacology 2022;13:937502

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Xiaojianzhong decoction attenuates gastric mucosal injury by activating the p62/Keap1/Nrf2 signaling pathway to inhibit ferroptosis.

Chen J, Zhang J, Chen T, Bao S, Li J, Wei H, Hu X, Liang Y, Liu F, Yan S
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2022 Nov;155:113631

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Iron-overloaded follicular fluid increases the risk of endometriosis-related infertility by triggering granulosa cell ferroptosis and oocyte dysmaturity.

Ni Z, Li Y, Song D, Ding J, Mei S, Sun S, Cheng W, Yu J, Zhou L, Kuang Y, Li M, Cai Z, Yu C
Cell death & disease 2022 Jul 4;13(7):579

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Tolerable upper intake level of iron damages the liver of weaned piglets.

Ding H, Zhao Y, Yu X, Chen L, Han J, Feng J
Journal of animal physiology and animal nutrition 2021 Jul;105(4):668-677

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of Ferritin expression in Jurkat cell lysate (M02401). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FTH1 monoclonal antibody (Catalog # M02401) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FTH1

Additional image