PA5-72811

antibody from Invitrogen Antibodies

Targeting: MFN2 CMT2A2, CPRP1, KIAA0214, MARF

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-72811

Provider

Invitrogen Antibodies

Product name

MFN2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Mutated FANCA Gene Role in the Modulation of Energy Metabolism and Mitochondrial Dynamics in Head and Neck Squamous Cell Carcinoma.

Bertola N, Degan P, Cappelli E, Ravera S
Cells 2022 Jul 30;11(15)

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The Role of Endoplasmic Reticulum in the Differential Endurance against Redox Stress in Cortical and Spinal Astrocytes from the Newborn SOD1(G93A) Mouse Model of Amyotrophic Lateral Sclerosis.

Marini C, Cossu V, Kumar M, Milanese M, Cortese K, Bruno S, Bellese G, Carta S, Zerbo RA, Torazza C, Bauckneht M, Venturi C, Raffa S, Orengo AM, Donegani MI, Chiola S, Ravera S, Castellani P, Morbelli S, Sambuceti G, Bonanno G
Antioxidants (Basel, Switzerland) 2021 Aug 30;10(9)

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Mechanisms underlying the predictive power of high skeletal muscle uptake of FDG in amyotrophic lateral sclerosis.

Marini C, Cossu V, Bonifacino T, Bauckneht M, Torazza C, Bruno S, Castellani P, Ravera S, Milanese M, Venturi C, Carlone S, Piccioli P, Emionite L, Morbelli S, Orengo AM, Donegani MI, Miceli A, Raffa S, Marra S, Signori A, Cortese K, Grillo F, Fiocca R, Bonanno G, Sambuceti G
EJNMMI research 2020 Jul 7;10(1):76

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of MFN2 in human brain tissue lysate with MFN2 Polyclonal Antibody (Product # PA5-72811) at 1 µg/mL.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of MFN2 in mouse brain tissue with MFN2 Polyclonal Antibody (Product # PA5-72811) at 20 µg/mL. Green: MFN2 Red: Phylloidin staining Blue: DAPI staining

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of MFN2 in rat brain tissue with MFN2 Polyclonal Antibody (Product # PA5-72811) at 20 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of MFN2 in mouse brain tissue with MFN2 Polyclonal Antibody (Product # PA5-72811) at 5 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of MFN2 in rat brain tissue with MFN2 Polyclonal Antibody (Product # PA5-72811) at 5 µg/mL.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6 Electron microscopy ultrastructure of ER and mitochondria. Mitochondrial number ( a ), mitochondria max diameter ( b ), ER surface ( c ) in wildtype (blue) and SOD1 G93A (red) of motor cortex (MC, circles) and spinal cord (SC, squares) astrocytes. Number of contact points ( d ) and distance between ER and mitochondria ( e ) evaluated in MC and SC astrocytes harvested from WT and SOD1 G93A mice (with the green area indicating distance < 30 nm, considered as contact points). Western blot analysis and relative densitometry quantitative analyses of Mfn2 ( f ) in wildtype (blue) and SOD1 G93A (red) astrocytes harvested from MC and SC. Data are shown as the mean +- SD. n = 3 experiments per group, with each value defined in triplicate. ** = p < 0.01, *** = p < 0.001 vs. corresponding MC astrocytes.