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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
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Validation data
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- Product number
- 711803 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MFN2 Recombinant Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Eye Opener in Stroke.
Nguyen H, Lee JY, Sanberg PR, Napoli E, Borlongan CV
Stroke 2019 Aug;50(8):2197-2206
Stroke 2019 Aug;50(8):2197-2206
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis (Fig a) of MFN2 was performed on cell extracts (100 µg of lysate) of U-2 OS wild type (Lane 1) and Mitofusin 2 knockout (Lane 2). The blot was probed with Anti-MFN2 Recombinant Rabbit Polyclonal Antibody (Product # 711803, 1:400 dilution) and detected by chemiluminescence using Peroxidase AffiniPure Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Product # 111-035-144, 1:4000 dilution). Densitometric analysis of this Western blot is shown in the histogram (Fig b). Loss of signal upon CRISPR mediated knockout (KO) confirms that antibody is specific to MFN2.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- For immunofluorescence analysis, SH-SY5Y cells were fixed and permeabilized for detection of endogenous Mitofusin 2 using Mitofusin 2 Recombinant Rabbit Polyclonal Antibody (Product # 711803, 5 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of Mitofusin 2 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents mitochondrial staining using MitoTracker® Red CMXRos (Product # M7512). Panel d) is a composite image of Panels a, b and c clearly demonstrating co-localization of Mitofusin 2 with mitotracker which specifically binds to the mitochondria. Panel e) represents control cells without primary antibody to assess background. The images were captured at 60X magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- For immunofluorescence analysis, SH-SY5Y cells were fixed and permeabilized for detection of endogenous Mitofusin 2 using Anti- Mitofusin 2 Recombinant Rabbit Polyclonal Antibody (Product # 711803, 5 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of Mitofusin 2 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating mitochondrial localization of Mitofusin 2. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
