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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MA5-16141 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Palladin Monoclonal Antibody (1E6)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Suggested positive control: MDA-MB231 (human breast cancer) lysates.
- Reactivity
- Human, Mouse, Rat, Canine, Chicken/Avian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1E6
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Palladin in HeLa (A), A431 (B), NIH3T3 (C), and PC-12 (D) cell line extracts . Samples were incubated in Palladin monoclonal antibody (Product # MA5-16141) using a dilution of 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Palladin in MDA-MB-231 lysate. Sample was incubated in Palladin monoclonal antibody (Product # MA5-16141).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of Palladin in a7r5 cells. Samples were incubated in Palladin monoclonal antibody (Product # MA5-16141).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Palladin in immersion fixed paraffin-embedded sections of human heart. Samples were incubated in Palladin monoclonal antibody (Product # MA5-16141) using a dilution of 1:100 for 1 hour at room temperature followed by the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in cardiomyocytes.
