PA5-96323

antibody from Invitrogen Antibodies

Targeting: MGP

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96323

Provider

Invitrogen Antibodies

Product name

MGP Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: YESHESMESY ELNPFINRRN ANTFISPQQR WRAKVQERIR ERSKPVHELN REACDDYRLC ERYAMVYGYN AAYNRYFRKR RGTK; Positive Samples: MCF7, U-87MG, A375, BT-474, 3T3L1, Mouse kidney, Mouse lung, Mouse heart

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.79 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MGP in extracts of SH-SY5Y cells. Samples were incubated with MGP Polyclonal antibody (Product # PA5-96323) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MGP in paraffin-embedded human breast cancer using. Samples were incubated with MGP Polyclonal antibody (Product # PA5-96323) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MGP in paraffin-embedded rat breast. Samples were incubated with MGP Polyclonal antibody (Product # PA5-96323) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Immunofluorescence) analysis of MGP in rat cartilage using. Samples were incubated with MGP Polyclonal antibody (Product # PA5-96323) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Immunofluorescence) analysis of MGP in mouse cartilage. Samples were incubated with MGP Polyclonal antibody (Product # PA5-96323) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Immunofluorescence) analysis of MGP in mouse cartilage. Samples were incubated with MGP Polyclonal antibody (Product # PA5-96323) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.