Explore
Validate
Learn
Western blot
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-35859 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATOH7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: ATOH7-transfected 293T. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Role of mTORC1 activity during early retinal development and lamination in human-induced pluripotent stem cell-derived retinal organoids.
Lee SH, Han JW, Yang JY, Jun HO, Bang JH, Shin H, Choi JH, Lee J, Madrakhimov SB, Chung KH, Chang HS, Lyu J, Park TK
Cell death discovery 2022 Feb 8;8(1):56
Cell death discovery 2022 Feb 8;8(1):56
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 1 Formation of hiPSC-derived ROs and RGC development at an early stage. A Main steps of hiPSC-derived RO development in vitro: hiPSC colony, EB formation, neuroretinal domain, and ROs. B Immunofluorescence staining of HuC/D (green) and AtoH7 (red) of ROs at distinct differentiated time points. The expression of HuC/D and AtoH7 was gradually increased in the innermost layers of ROs from day 35 to day 60. C Quantitative RT-PCR results of PAX6, AtoH7, Islet-1, Brn3b, and Tuj1 levels of ROs at indicated time points. Data are expressed by mean +- S.E.M. * p < 0.05, ** p < 0.01 by the Kruskal-Wallis test with post hoc analysis. Scale bar, 50 mum.
