14649-1-AP

antibody from Invitrogen Antibodies

Targeting: SH3RF1 KIAA1494, POSH, RNF142, SH3MD2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14649-1-AP

Provider

Invitrogen Antibodies

Product name

POSH Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: VTMVSPSTA GGPAQKLQGN GVAGSPSVVP AAVVSAAHIQ TSPQAKVLLH MTGQMTVNQA RNAVRTVAAH NQERPTAAVT PIQVQNAAGL SPASVGLSHH SLASPQPAPL MPGSATHTAA ISISRASAPL ACAAAAPLTS PSITSASLEA EPSGRIVTVL PGLPTSPDSA SSACGNSSAT KPDKDSKKEK KGLLKLLSGA STKRKPRVSP PASPTLEVEL GSAELPLQGA VGPELPPGGG HGRAGSCPVD GDGPVTTAVA GAALAQDAFH RKASSLDSAV PIAPPPRQAC SSLGPVLNES RPVVCERHRV VVSYPPQSEA ELELKEGDIV FVHKKREDGW FKGTLQRNGK TGLFPGSFVE NI (696-1056 aa encoded by BC053671)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.23 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 14649-1-AP (POSH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 14649-1-AP (POSH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

DU 145 cells were subjected to SDS PAGE followed by western blot with 14649-1-AP (POSH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 14649-1-AP (POSH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 14649-1-AP (POSH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 14649-1-AP (POSH antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 14649-1-AP (POSH antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 14649-1-AP ( POSH antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 14649-1-AP ( POSH antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-POSH (IP:14649-1-AP, 4ug; Detection:14649-1-AP 1:1000) with mouse brain tissue lysate 4000ug.