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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoprecipitation [1]
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Validation data
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- Product number
- MA5-55676 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SH3RF1 Monoclonal Antibody (K1E030_5E8)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Sequence of this protein is as follows: GNSSATKPDK DSKKEKKGLL KLLSGASTKR KPRVSPPASP TLEVELGSAE LPLQGAVGPE LPPGGGHGRA GSCPVDGDGP VTTAVAGAAL AQDAFHRKAS SLDSAVPIAP PPRQACSSLG PVLNESRPVV CERHRVVVSY PPQSEAELEL KEGDIVFVHK KREDGWFKGT LQRNGKTGLF
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- K1E030_5E8
- Vial size
- 50 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunoprecipitation of SH3RF1 in 200 µg of mouse brain tissue lysate. Samples are as follows: Lane 1: Mouse brain tissue lysate, Lane 2: SH3RF1 immunoprecipitated from mouse brain tissue lysate, Lane3: The same as Lane 2 but KT82 was used as IgG isotype control antibody. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE, blotted onto nitrocellulose membrane, and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. The isotype control antibody was KT82. Incubation of samples with SH3RF1 monoclonal antibody (Product # MA5-55676) at a dilution of 2.5 µg was used.
