PAB23481

antibody from Abnova Corporation

Targeting: NEK1 KIAA1901, NY-REN-55

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB23481

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB23481, RRID:AB_11121199

Product name

NEK1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant NEK1.

Antigen sequence

VLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDV
SPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDS
SLTDTRETSEEMQKTNNAISSKR


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with NEK1 polyclonal antibody (Cat # PAB23481) at 1-4 ug/mL dilution shows positivity in nuclear membrane.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human breast with NEK1 polyclonal antibody (Cat # PAB23481) shows strong cytoplasmic and membranous positivity in glandular cells at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)