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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunoprecipitation [1]
- Immunohistochemistry [4]
- Other assay [1]
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- Product number
- PA5-54271 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NEK1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Immunogen sequence: NLKAQEDEKG KQNLSDTFEI NVHEDAKEHE KEKSVSSDRK KWEAGGQLVI PLDELTLDTS FSTTERHTVG EVIKLGPNGS PRRAWGKSPT DSVLKILGEA ELQLQTELLE NTTIRSEISP EGEKYKPLIT GEKKVQCISH EINPSA Highest antigen sequence identity to the following orthologs: Mouse - 72%, Rat - 71%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.7 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of NEK1 was performed on HeLa cell lysates. Antibody-bead conjugates were prepared by adding 1 µg of NEK1 polyclonal antibody (Product # PA5-54271) with 30 µL of protein A -Sepharose beads and rocked overnight at 4 deg. 1 µg of lysate was incubated with antibody-bead conjugate for 2 hrs at 4 deg. After multiple washes, 10% starting material (SM), 10% unbound fraction (UB) and immunoprecipitated fraction (IP) were processed for immunoblot using a different NEK1 polyclonal antibody (Product # PA5-78074). Ponceau stained transfer of blot is shown. Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NEK1 in human endometrium using NEK1 Polyclonal Antibody (Product # PA5-54271) shows moderate membranous positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NEK1 in human Fallopian tube using NEK1 Polyclonal Antibody (Product # PA5-54271) shows strong cytoplasmic/ membranous positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NEK1 in human skin using NEK1 Polyclonal Antibody (Product # PA5-54271) shows weak cytoplasmic positivity in squamous epithelial cells as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NEK1 in human testis using NEK1 Polyclonal Antibody (Product # PA5-54271) shows strong cytoplasmic positivity in cells in seminiferous ducts.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of NEK1 was performed on HeLa cell lysates. Antibody-bead conjugates were prepared by adding 1 µg of NEK1 polyclonal antibody (Product # PA5-54271) with 30 µL of protein A -Sepharose beads and rocked overnight at 4 deg. 1 µg of lysate was incubated with antibody-bead conjugate for 2 hrs at 4 deg. After multiple washes, 10% starting material (SM), 10% unbound fraction (UB) and immunoprecipitated fraction (IP) were processed for immunoblot using a different NEK1 polyclonal antibody (Product # PA5-78074). Ponceau stained transfer of blot is shown. Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.
