702272

antibody from Invitrogen Antibodies

Targeting: LAMA4 LAMA3

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

702272

Provider

Invitrogen Antibodies

Product name

Laminin-221 Recombinant Rabbit Monoclonal Antibody (17H4L1)

Antibody type

Monoclonal

Antigen

Other

Description

This antibody is predicted to react with Monkey, Sheep, Cat and Bovine

Antibody clone number

17H4L1

Concentration

0.5 mg/mL

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis was performed on fixed and permeabilized C2C12 cells for detection of Laminin 221 using Anti-Laminin Recombinant Rabbit Monoclonal Antibody (Product # 702272, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of Laminin 221 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating localization of Laminin 221 in the extracellular matrix. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.