MA5-24650

antibody from Invitrogen Antibodies

Targeting: LAMA4 LAMA3

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-24650

Provider

Invitrogen Antibodies

Product name

Laminin alpha-4 Monoclonal Antibody (CL3183)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: ENLLNQAREL QAKAESSSDE AVADTSRRVG GALARKSALK TRLSDAVKQL QAAERGDAQQ RLGQSRLITE EANRTTMEVQ QATAPMANNL TNWSQNLQHF DSSAYNTAVN SARDAVRNLT EVVPQLLDQL RTVEQKRPAS Highest antigen sequence identity to the following orthologs: Mouse - 86%, Rat - 85%. Binds to an epitope located within the peptide sequence MANNLTNWSQNLQHF as determined by overlapping synthetic peptides.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

CL3183

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Laminin-α4 Is Upregulated in Both Human and Murine Models of Obesity.

Goddi A, Carmona A, Schroedl L, White JM, Piron MJ, De Leon A, Casimiro I, Hoffman A, Gonzalez Porras MA, Brey EM, Brady MJ, Cohen RN
Frontiers in endocrinology 2021;12:698621

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Laminin alpha-4 in human colon using Laminin alpha-4 Monoclonal Antibody (CL3183) (Product # MA5-24650) shows strong positivity in basement membrane of glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Laminin alpha-4 in human placenta using Laminin alpha-4 Monoclonal Antibody (CL3183) (Product # MA5-24650) shows strong positivity in basement membrane of trophoblastic cells and endothelial cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Laminin alpha-4 in human prostate using Laminin alpha-4 Monoclonal Antibody (CL3183) (Product # MA5-24650) shows moderate to strong membranous positivity in smooth muscle cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Laminin alpha-4 in mouse stomach using Laminin alpha-4 Monoclonal Antibody (CL3183) (Product # MA5-24650) shows moderate positivity in basement membrane of glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Laminin alpha-4 in rat kidney using Laminin alpha-4 Monoclonal Antibody (CL3183) (Product # MA5-24650) shows strong membranous positivity in cells in glomeruli.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Laminin alpha-4 in rat stomach using Laminin alpha-4 Monoclonal Antibody (CL3183) (Product # MA5-24650) shows moderate to strong positivity in basement membrane of glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Laminin alpha-4 in human lymph node using Laminin alpha-4 Monoclonal Antibody (CL3183) (Product # MA5-24650) shows no positivity in non-germinal center cells as expected.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Laminin alpha-4 in mouse epididymis using Laminin alpha-4 Monoclonal Antibody (CL3183) (Product # MA5-24650) shows moderate to strong membranous positivity in smooth muscle cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Laminin alpha-4 in rat heart muscle using Laminin alpha-4 Monoclonal Antibody (CL3183) (Product # MA5-24650) shows moderate to strong membranous positivity in cardiomyocytes.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Gene expression of laminin-alpha chains in sWAT of HFD fed mice. (A) Average weekly weights of mice on regular chow diet (RCD) and 45% High Fat Diet (HFD) for 8 weeks. RCD (n=7), HFD (n=8). (*,**,***) indicates p < 0.05, 0.01, 0.001, respectively. Data are Means +- SD. (B) Laminin-alpha chain mRNA expression in sWAT of mice after 8 weeks of dietary study normalized to the average of RCD control group. RCD (n=7), HFD (n=8). (C) Relative Lama2 mRNA expression normalized to RCD group graphed against weight (grams) for each mouse in 8-week dietary study (n=15). Simple linear regression analysis. (D) Relative Lama4 mRNA expression graphed against weight (grams) for each mouse in 8-week dietary study (n=15). Simple linear regression analysis. (E) Relative Lama4 mRNA expression graphed against adipocyte area (um 2 ) for one cohort of HFD and RCD mice (n=6). Simple linear regression analysis. (F) Representative images of H&E stained sWAT tissue sections of HFD and RCD mice after 8 weeks of dietary study. All images are 40X magnification. (G) Protein expression of LAMA4 and loading control B-actin in sWAT of mice placed on RCD or 45% HFD for 8 weeks was assessed by western blot. Original blot images can be found in the supplementary file. (H) LAMA4 signal from the western blot was quantified and normalized to the loading control signal. Values are shown as fold changes in comparison to the average of the RCD group. RCD (n=3), HFD (n=5).