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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- Ab119724 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- MAPK11 Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit anti Human MAPK11 Antibody, Polyclonal antibody, could be used for WB, IHC and so on.ApplicationWB: 1/500 - 1/1000 IHC: 1/50 - 1/100Protein FunctionKinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment, by cytokines, or by environmental stress. Phosphorylates preferentially transcription factor ATF2.Post-translationalDually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 251-364- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,6 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- MAPK11 Antibody (Ab119724) - Western Blot All lanes: MAPK11 Antibody (Ab119724) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution Predicted band size: 41 kDa Observed band size: 38 kDa Exposure time: 60.0 s
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- MAPK11 Antibody (Ab119724) - Western Blot All lanes: MAPK11 Antibody (Ab119724) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 42 kDa Observed band size: 39 kDa Exposure time: 100.0 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- MAPK11 Antibody (Ab119724) - IHC Immunohistochemistry analysis of paraffin-embedded Human brain using MAPK11 Antibody (Ab119724). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
