PA1-41154

antibody from Invitrogen Antibodies

Targeting: MAPK11 p38-2, p38Beta, PRKM11, SAPK2

Western blot (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-41154

Provider

Invitrogen Antibodies

Product name

p38 MAPK beta Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Suggested positive control: human brain tissue, human brain protein.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 μg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Regulation of Sirtuin-3 and ERK1/2/p38MAPK by the combination Ga nanoparticles/γ-radiation low dosage: an effective approach for treatment of hepatocellular carcinoma.

Abdalla MS, El-Mahdy EM, Mansour SZ, Elsonbaty SM, Amin MH
Journal, genetic engineering & biotechnology 2022 Jul 1;20(1):93

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Inhibiting p38 MAPK alpha rescues axonal retrograde transport defects in a mouse model of ALS.

Gibbs KL, Kalmar B, Rhymes ER, Fellows AD, Ahmed M, Whiting P, Davies CH, Greensmith L, Schiavo G
Cell death & disease 2018 May 22;9(6):596

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 4 p38 MAPKalpha knockdown normalises axonal retrograde transport in SOD1 G93A motor neurons. a Western blot analysis reveals all four p38 MAPK isoforms (alpha, beta, gamma and delta) are expressed in wild type and SOD1 G93A motor neuron lysates. Mouse brain lysate was loaded as an antibody control for p38 MAPKbeta. The upper band is only detected in spinal cord extracts and may represent a posttranslational modified form of p38 MAPKbeta. b Lentiviral delivery of shRNA targeted against p38 MAPKalpha reduces its levels in primary motor neurons. Transduced motor neurons express GFP. c Speed profiles of H C T-containing signalling endosomes in wild-type motor neurons transduced with scrambled shRNA (black squares), SOD1 G93A motor neurons transduced with scrambled shRNA (red diamonds) and SOD1 G93A motor neurons transduced with p38 MAPKalpha shRNA (blue triangles). Axonal transport has been quantified only in GFP-positive motor neurons. Comparison of the curves reveals that knocking down p38 MAPKalpha in SOD1 G93A motor neurons (blue triangles) accelerates axonal transport to wild-type speeds (black squares) (wild type + scrambled shRNA: 89 carriers, 17 axons; SOD1 G93A + scrambled shRNA: 104 carriers, 24 axons; SOD1 G93A + p38 MAPKalpha shRNA: 99 carriers, 20 axons; 4 independent experiments). d Lentiviral delivery of scrambled shRNA has no effect on levels of p38 MAPKalpha in primary motor neurons. Transduced motor neurons express GFP. e Speed profiles of H