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LearnA301-472A
antibody from Invitrogen Antibodies
Targeting: MED24
CRSP100, CRSP4, DRIP100, KIAA0130, MED5, THRAP4, TRAP100
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Other assay [1]
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- Product number
- A301-472A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRAP100/MED24 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human TRAP100/MED24 by western blot. Samples: Whole cell lysate (50 µg) from RKO, GaMG, HEK293T, LNCaP, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRAP100/MED24 antibody (Product # A301-472A lot 2) used for WB at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse TRAP100/MED24 by western blot. Samples: Whole cell lysate (25 µg) from NIH 3T3, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRAP100/MED24 antibody (Product # A301-472A lot 2) used for WB at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse TRAP100/MED24 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate (50 µg for WB) from MCF7 (7), PANC1 (P), 293T (T) and mouse NIH3T3 (M) cells. For IP, whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells was used. Antibody: Affinity purified rabbit anti-TRAP100/MED24 antibody A301-472A used for WB at 1 µg/ml (A and B) and used for IP at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human TRAP100/MED24 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRAP100/MED24 antibody (Product # A301-472A; Lot 2) used for IP at 6 µg per reaction. TRAP100/MED24 was also immunoprecipitated by a previous lot of this antibody (Product # A301-472A; Lot 1). For blotting immunoprecipitated TRAP100/MED24, Product # A301-472A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
