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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- H00001879-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00001879-M01, RRID:AB_425406
- Product name
- EBF1 monoclonal antibody (M01), clone 1C12
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant EBF1.
- Antigen sequence
MFGIQESIQRSGSSMKEEPLGSGMNAVRTWMQGAG
VLDANTAAQSGVGLARAHFEKQPPSNLRKSNFFHF
VLALYDRQGQPVEIERTAFVGFVEKEKEANSEKTN
NGIHYRLQLLYSNGIRTEQDFYVRLIDSMTKQAIV
YEGQDKNPEMCRVLLTHEIMCSRCCDKKSCGNRNE
TPSDPVIIDRFFSKFFLKCNQNCLKNAGNPRDMRR
FQVVVSTTVNVDGHVLAVSDNMFVHNNSKHGRRAR
RLDPSEGTPSYLEHATPCIKAISPSEGWTTGGATV
IIIGDNFFDGLQVIFGTMLVWSELITPHAIRVQTP
PRHIPGVVEVTLSYKSKQFCKGTPGRFIYTALNEP
TIDYGFQRLQKVIPRHPGDPERLPKEVILKRAADL
VEALYGMPHNNQEIILKRAADIAEALYSVPRNHNQ
LPALANTSVHAGMMGVNSFSGQLAVNVSEASQATN
QGFTRNSSSVSPHGYVPSTTPQQTNYNSVTTSMNG
YGSAAMSNLGGSPTFLNGSAANSPYAIVPSSPTMA
SSTSLPSNCSSSSGIFSFSPANMVSAVKQKSAFAP
VVRPQTSPPPTCTSTNGNSLQAISGMIVPPM- Isotype
- IgG
- Antibody clone number
- 1C12
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Early B-cell factor-1 (EBF1) is a key regulator of metabolic and inflammatory signaling pathways in mature adipocytes.
Cellular origin and pathophysiology of chronic lymphocytic leukemia.
Griffin MJ, Zhou Y, Kang S, Zhang X, Mikkelsen TS, Rosen ED
The Journal of biological chemistry 2013 Dec 13;288(50):35925-39
The Journal of biological chemistry 2013 Dec 13;288(50):35925-39
Cellular origin and pathophysiology of chronic lymphocytic leukemia.
Seifert M, Sellmann L, Bloehdorn J, Wein F, Stilgenbauer S, Dürig J, Küppers R
The Journal of experimental medicine 2012 Nov 19;209(12):2183-98
The Journal of experimental medicine 2012 Nov 19;209(12):2183-98
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of EBF1 expression in transfected 293T cell line by EBF1 monoclonal antibody (M01), clone 1C12.Lane 1: EBF1 transfected lysate(64 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged EBF1 is 0.1 ng/ml as a capture antibody.
