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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- 701316 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RAGE Recombinant Rabbit Monoclonal Antibody (17H19L10)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 17H19L10
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RAGE in whole cell extracts from MDA-MB-231 cells using a RAGE recombinant rabbit monoclonal antibody (Product # 701316) at a dilution of 2 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~32kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of RAGE in HeLa cells using a RAGE recombinant rabbit monoclonal antibody (Product # 701316). Cells were fixed and permeabilized using FIX & PERM (Product # GAS004) reagent, and detection was performed using an Alexa Fluor 488 goat anti-rabbit IgG (right peak) compared to an isotype control (left peak).
