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- Antibody Data
- Antigen structure
- References [1]
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- Western blot [1]
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- Product number
- MAB11792 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal AGER Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse RAGE in ELISAs and Western blots.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 500 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Receptor for advanced glycation end-products (RAGE) is an indicator of direct lung injury in models of experimental lung injury.
Su X, Looney MR, Gupta N, Matthay MA
American journal of physiology. Lung cellular and molecular physiology 2009 Jul;297(1):L1-5
American journal of physiology. Lung cellular and molecular physiology 2009 Jul;297(1):L1-5
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Mouse RAGE by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 2 µg/mL of Mouse RAGE Monoclonal Antibody (Catalog # MAB11792) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for RAGE and mature RAGE at approximately 50 kDa and 38 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
