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- Antibody Data
- Antigen structure
- References [9]
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- Validations
- Western blot [1]
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- Product number
- MAB11451-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human RAGE Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human RAGE in ELISAs and Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse RAGE and recombinant rat RAGE is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q15109- Isotype
- IgG
- Antibody clone number
- 176902
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Transactivation of RAGE mediates angiotensin-induced inflammation and atherogenesis.
Claudin-18-mediated YAP activity regulates lung stem and progenitor cell homeostasis and tumorigenesis.
High-density lipoproteins suppress Aβ-induced PBMC adhesion to human endothelial cells in bioengineered vessels and in monoculture.
Autophagy-Lysosome Pathway in Renal Tubular Epithelial Cells Is Disrupted by Advanced Glycation End Products in Diabetic Nephropathy.
Soluble receptor for advanced glycation end products as an indicator of pulmonary vascular injury after cardiac surgery.
Proteolytic release of the receptor for advanced glycation end products from in vitro and in situ alveolar epithelial cells.
Effects of atorvastatin on serum soluble receptors for advanced glycation end-products in type 2 diabetes.
S100A11, an dual mediator for growth regulation of human keratinocytes.
Receptor for advanced glycation endproducts mediates neutrophil migration across intestinal epithelium.
Pickering RJ, Tikellis C, Rosado CJ, Tsorotes D, Dimitropoulos A, Smith M, Huet O, Seeber RM, Abhayawardana R, Johnstone EK, Golledge J, Wang Y, Jandeleit-Dahm KA, Cooper ME, Pfleger KD, Thomas MC
The Journal of clinical investigation 2019 Jan 2;129(1):406-421
The Journal of clinical investigation 2019 Jan 2;129(1):406-421
Claudin-18-mediated YAP activity regulates lung stem and progenitor cell homeostasis and tumorigenesis.
Zhou B, Flodby P, Luo J, Castillo DR, Liu Y, Yu FX, McConnell A, Varghese B, Li G, Chimge NO, Sunohara M, Koss MN, Elatre W, Conti P, Liebler JM, Yang C, Marconett CN, Laird-Offringa IA, Minoo P, Guan K, Stripp BR, Crandall ED, Borok Z
The Journal of clinical investigation 2018 Mar 1;128(3):970-984
The Journal of clinical investigation 2018 Mar 1;128(3):970-984
High-density lipoproteins suppress Aβ-induced PBMC adhesion to human endothelial cells in bioengineered vessels and in monoculture.
Robert J, Button EB, Stukas S, Boyce GK, Gibbs E, Cowan CM, Gilmour M, Cheng WH, Soo SK, Yuen B, Bahrabadi A, Kang K, Kulic I, Francis G, Cashman N, Wellington CL
Molecular neurodegeneration 2017 Aug 22;12(1):60
Molecular neurodegeneration 2017 Aug 22;12(1):60
Autophagy-Lysosome Pathway in Renal Tubular Epithelial Cells Is Disrupted by Advanced Glycation End Products in Diabetic Nephropathy.
Liu WJ, Shen TT, Chen RH, Wu HL, Wang YJ, Deng JK, Chen QH, Pan Q, Huang Fu CM, Tao JL, Liang D, Liu HF
The Journal of biological chemistry 2015 Aug 14;290(33):20499-510
The Journal of biological chemistry 2015 Aug 14;290(33):20499-510
Soluble receptor for advanced glycation end products as an indicator of pulmonary vascular injury after cardiac surgery.
Tuinman PR, Cornet AD, Kuipers MT, Vlaar AP, Schultz MJ, Beishuizen A, Groeneveld AB, Juffermans NP
BMC pulmonary medicine 2013 Dec 16;13:76
BMC pulmonary medicine 2013 Dec 16;13:76
Proteolytic release of the receptor for advanced glycation end products from in vitro and in situ alveolar epithelial cells.
Yamakawa N, Uchida T, Matthay MA, Makita K
American journal of physiology. Lung cellular and molecular physiology 2011 Apr;300(4):L516-25
American journal of physiology. Lung cellular and molecular physiology 2011 Apr;300(4):L516-25
Effects of atorvastatin on serum soluble receptors for advanced glycation end-products in type 2 diabetes.
Tam HL, Shiu SW, Wong Y, Chow WS, Betteridge DJ, Tan KC
Atherosclerosis 2010 Mar;209(1):173-7
Atherosclerosis 2010 Mar;209(1):173-7
S100A11, an dual mediator for growth regulation of human keratinocytes.
Sakaguchi M, Sonegawa H, Murata H, Kitazoe M, Futami J, Kataoka K, Yamada H, Huh NH
Molecular biology of the cell 2008 Jan;19(1):78-85
Molecular biology of the cell 2008 Jan;19(1):78-85
Receptor for advanced glycation endproducts mediates neutrophil migration across intestinal epithelium.
Zen K, Chen CX, Chen YT, Wilton R, Liu Y
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 15;178(4):2483-90
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 15;178(4):2483-90
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human RAGE by Western Blot. Western blot shows lysates of human lung tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human RAGE Monoclonal Antibody (Catalog # MAB11451) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for RAGE at approximately 43 kDa under non-reducing (NR) conditions and 50 kDa under reducing (R) conditions (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
