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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [11]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MAB1179-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse/Rat RAGE Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse and rat RAGE in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human RAGE is observed.
- Reactivity
- Mouse, Rat
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
NP_031451- Isotype
- IgG
- Antibody clone number
- 175410
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references IL-1 and TNFα Contribute to the Inflammatory Niche to Enhance Alveolar Regeneration.
RAGE-specific single chain Fv for PET imaging of pancreatic cancer.
Pulmonary pericytes regulate lung morphogenesis.
Therapeutic Effectiveness of Anti-RAGE Antibody Administration in a Rat Model of Crush Injury.
Recruited Monocytes and Type 2 Immunity Promote Lung Regeneration following Pneumonectomy.
The development and plasticity of alveolar type 1 cells.
Two nested developmental waves demarcate a compartment boundary in the mouse lung.
Alveolar progenitor and stem cells in lung development, renewal and cancer.
Receptor for advanced glycation end products (RAGE) functions as receptor for specific sulfated glycosaminoglycans, and anti-RAGE antibody or sulfated glycosaminoglycans delivered in vivo inhibit pulmonary metastasis of tumor cells.
Aldose reductase and AGE-RAGE pathways: central roles in the pathogenesis of vascular dysfunction in aging rats.
Role of soluble receptor for advanced glycation end products on endotoxin-induced lung injury.
Katsura H, Kobayashi Y, Tata PR, Hogan BLM
Stem cell reports 2019 Apr 9;12(4):657-666
Stem cell reports 2019 Apr 9;12(4):657-666
RAGE-specific single chain Fv for PET imaging of pancreatic cancer.
Kim HY, Wang X, Kang R, Tang D, Boone BA, Zeh HJ 3rd, Lotze MT, Edwards WB
PloS one 2018;13(3):e0192821
PloS one 2018;13(3):e0192821
Pulmonary pericytes regulate lung morphogenesis.
Kato K, Diéguez-Hurtado R, Park DY, Hong SP, Kato-Azuma S, Adams S, Stehling M, Trappmann B, Wrana JL, Koh GY, Adams RH
Nature communications 2018 Jun 22;9(1):2448
Nature communications 2018 Jun 22;9(1):2448
Therapeutic Effectiveness of Anti-RAGE Antibody Administration in a Rat Model of Crush Injury.
Matsumoto H, Matsumoto N, Shimazaki J, Nakagawa J, Imamura Y, Yamakawa K, Yamada T, Ikeda M, Hiraike H, Ogura H, Shimazu T
Scientific reports 2017 Sep 25;7(1):12255
Scientific reports 2017 Sep 25;7(1):12255
Recruited Monocytes and Type 2 Immunity Promote Lung Regeneration following Pneumonectomy.
Lechner AJ, Driver IH, Lee J, Conroy CM, Nagle A, Locksley RM, Rock JR
Cell stem cell 2017 Jul 6;21(1):120-134.e7
Cell stem cell 2017 Jul 6;21(1):120-134.e7
The development and plasticity of alveolar type 1 cells.
Yang J, Hernandez BJ, Martinez Alanis D, Narvaez del Pilar O, Vila-Ellis L, Akiyama H, Evans SE, Ostrin EJ, Chen J
Development (Cambridge, England) 2016 Jan 1;143(1):54-65
Development (Cambridge, England) 2016 Jan 1;143(1):54-65
Two nested developmental waves demarcate a compartment boundary in the mouse lung.
Alanis DM, Chang DR, Akiyama H, Krasnow MA, Chen J
Nature communications 2014 May 29;5:3923
Nature communications 2014 May 29;5:3923
Alveolar progenitor and stem cells in lung development, renewal and cancer.
Desai TJ, Brownfield DG, Krasnow MA
Nature 2014 Mar 13;507(7491):190-4
Nature 2014 Mar 13;507(7491):190-4
Receptor for advanced glycation end products (RAGE) functions as receptor for specific sulfated glycosaminoglycans, and anti-RAGE antibody or sulfated glycosaminoglycans delivered in vivo inhibit pulmonary metastasis of tumor cells.
Mizumoto S, Takahashi J, Sugahara K
The Journal of biological chemistry 2012 Jun 1;287(23):18985-94
The Journal of biological chemistry 2012 Jun 1;287(23):18985-94
Aldose reductase and AGE-RAGE pathways: central roles in the pathogenesis of vascular dysfunction in aging rats.
Hallam KM, Li Q, Ananthakrishnan R, Kalea A, Zou YS, Vedantham S, Schmidt AM, Yan SF, Ramasamy R
Aging cell 2010 Oct;9(5):776-84
Aging cell 2010 Oct;9(5):776-84
Role of soluble receptor for advanced glycation end products on endotoxin-induced lung injury.
Zhang H, Tasaka S, Shiraishi Y, Fukunaga K, Yamada W, Seki H, Ogawa Y, Miyamoto K, Nakano Y, Hasegawa N, Miyasho T, Maruyama I, Ishizaka A
American journal of respiratory and critical care medicine 2008 Aug 15;178(4):356-62
American journal of respiratory and critical care medicine 2008 Aug 15;178(4):356-62
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse RAGE by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse/Rat RAGE Monoclonal Antibody (Catalog # 1179) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for RAGE and mature RAGE at approximately 50 and 38 kDa, respectively (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- RAGE in Mouse Lung. RAGE was detected in perfusion fixed frozen sections of adult mouse lung using Rat Anti-Mouse/Rat RAGE Monoclonal Antibody (Catalog # MAB1179) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Northern-Lights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
