PA5-24787

antibody from Invitrogen Antibodies

Targeting: AGER RAGE, SCARJ1

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-24787

Provider

Invitrogen Antibodies

Product name

RAGE Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

4-Acetylantroquinonol B enhances cell death and inhibits autophagy by downregulating the PI3K/Akt/MDR1 pathway in gemcitabine-resistant pancreatic cancer cells.

Chen YY, Chen SY, Li TJ, Lin TW, Chen CC, Yen GC
Oncology letters 2022 Apr;23(4):128

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of U251 cells using an AGER polyclonal antibody (Product # PA5-24787) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6. 4-AAQB treatment downregulates HMGB1, RAGE and MDR1 protein expression. (A) The expression of HMGB1 and RAGE was analyzed using western blotting. (B and C) Semi-quantification of HMGB1 and RAGE protein levels. (D) The expression of MDR1 was analyzed using western blotting. (E) Semi-quantification of MDR1 protein levels. *P