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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- PA5-24787 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RAGE Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references 4-Acetylantroquinonol B enhances cell death and inhibits autophagy by downregulating the PI3K/Akt/MDR1 pathway in gemcitabine-resistant pancreatic cancer cells.
Chen YY, Chen SY, Li TJ, Lin TW, Chen CC, Yen GC
Oncology letters 2022 Apr;23(4):128
Oncology letters 2022 Apr;23(4):128
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using an AGER polyclonal antibody (Product # PA5-24787) in mouse lung tissue lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human lung carcinoma using an AGER polyclonal antibody (Product # PA5-24787), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of U251 cells using an AGER polyclonal antibody (Product # PA5-24787) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6. 4-AAQB treatment downregulates HMGB1, RAGE and MDR1 protein expression. (A) The expression of HMGB1 and RAGE was analyzed using western blotting. (B and C) Semi-quantification of HMGB1 and RAGE protein levels. (D) The expression of MDR1 was analyzed using western blotting. (E) Semi-quantification of MDR1 protein levels. *P
