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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [17]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- AF1145 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat RAGE/AGER Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human RAGE in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity recombinant canine RAGE is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q15109- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Modulation of soluble receptor for advanced glycation end-products (RAGE) isoforms and their ligands in healthy aging.
EMC3 coordinates surfactant protein and lipid homeostasis required for respiration.
The Role of Neutrophil Proteins on the Amyloid Beta-RAGE Axis.
The Mouse-Specific Splice Variant mRAGE_v4 Encodes a Membrane-Bound RAGE That Is Resistant to Shedding and Does Not Contribute to the Production of Soluble RAGE.
S100A8/A9 stimulates keratinocyte proliferation in the development of squamous cell carcinoma of the skin via the receptor for advanced glycation-end products.
Necrotic cell-derived high mobility group box 1 attracts antigen-presenting cells but inhibits hepatocyte growth factor-mediated tropism of mesenchymal stem cells for apoptotic cell death.
Expression of RAGE and HMGB1 in thymic epithelial tumors, thymic hyperplasia and regular thymic morphology.
The receptor for advanced glycation end-products (RAGE) is only present in mammals, and belongs to a family of cell adhesion molecules (CAMs).
Antenatal exposure of maternal secondhand smoke (SHS) increases fetal lung expression of RAGE and induces RAGE-mediated pulmonary inflammation.
Inflammation and pancreatic cancer: molecular and functional interactions between S100A8, S100A9, NT-S100A8 and TGFβ1.
Receptor for advanced glycation end products regulates adipocyte hypertrophy and insulin sensitivity in mice: involvement of Toll-like receptor 2.
In vitro effects of atorvastatin on lipopolysaccharide-induced gene expression in endometriotic stromal cells.
Low anticoagulant heparin targets multiple sites of inflammation, suppresses heparin-induced thrombocytopenia, and inhibits interaction of RAGE with its ligands.
Differentiated human alveolar epithelial cells and reversibility of their phenotype in vitro.
Receptor for advanced glycation endproducts mediates neutrophil migration across intestinal epithelium.
Receptor for advanced glycation endproducts mediates neutrophil migration across intestinal epithelium.
Distribution of the receptor for advanced glycation end products in the human male reproductive tract: prevalence in men with diabetes mellitus.
Scavello F, Zeni F, Tedesco CC, Mensà E, Veglia F, Procopio AD, Bonfigli AR, Olivieri F, Raucci A
Aging 2019 Mar 23;11(6):1648-1663
Aging 2019 Mar 23;11(6):1648-1663
EMC3 coordinates surfactant protein and lipid homeostasis required for respiration.
Tang X, Snowball JM, Xu Y, Na CL, Weaver TE, Clair G, Kyle JE, Zink EM, Ansong C, Wei W, Huang M, Lin X, Whitsett JA
The Journal of clinical investigation 2017 Dec 1;127(12):4314-4325
The Journal of clinical investigation 2017 Dec 1;127(12):4314-4325
The Role of Neutrophil Proteins on the Amyloid Beta-RAGE Axis.
Stock AJ, Kasus-Jacobi A, Wren JD, Sjoelund VH, Prestwich GD, Pereira HA
PloS one 2016;11(9):e0163330
PloS one 2016;11(9):e0163330
The Mouse-Specific Splice Variant mRAGE_v4 Encodes a Membrane-Bound RAGE That Is Resistant to Shedding and Does Not Contribute to the Production of Soluble RAGE.
Di Maggio S, Gatti E, Liu J, Bertolotti M, Fritz G, Bianchi ME, Raucci A
PloS one 2016;11(9):e0153832
PloS one 2016;11(9):e0153832
S100A8/A9 stimulates keratinocyte proliferation in the development of squamous cell carcinoma of the skin via the receptor for advanced glycation-end products.
Iotzova-Weiss G, Dziunycz PJ, Freiberger SN, Läuchli S, Hafner J, Vogl T, French LE, Hofbauer GF
PloS one 2015;10(3):e0120971
PloS one 2015;10(3):e0120971
Necrotic cell-derived high mobility group box 1 attracts antigen-presenting cells but inhibits hepatocyte growth factor-mediated tropism of mesenchymal stem cells for apoptotic cell death.
Vogel S, Börger V, Peters C, Förster M, Liebfried P, Metzger K, Meisel R, Däubener W, Trapp T, Fischer JC, Gawaz M, Sorg RV
Cell death and differentiation 2015 Jul;22(7):1219-30
Cell death and differentiation 2015 Jul;22(7):1219-30
Expression of RAGE and HMGB1 in thymic epithelial tumors, thymic hyperplasia and regular thymic morphology.
Moser B, Janik S, Schiefer AI, Müllauer L, Bekos C, Scharrer A, Mildner M, Rényi-Vámos F, Klepetko W, Ankersmit HJ
PloS one 2014;9(4):e94118
PloS one 2014;9(4):e94118
The receptor for advanced glycation end-products (RAGE) is only present in mammals, and belongs to a family of cell adhesion molecules (CAMs).
Sessa L, Gatti E, Zeni F, Antonelli A, Catucci A, Koch M, Pompilio G, Fritz G, Raucci A, Bianchi ME
PloS one 2014;9(1):e86903
PloS one 2014;9(1):e86903
Antenatal exposure of maternal secondhand smoke (SHS) increases fetal lung expression of RAGE and induces RAGE-mediated pulmonary inflammation.
Winden DR, Barton DB, Betteridge BC, Bodine JS, Jones CM, Rogers GD, Chavarria M, Wright AJ, Jergensen ZR, Jimenez FR, Reynolds PR
Respiratory research 2014 Oct 23;15(1):129
Respiratory research 2014 Oct 23;15(1):129
Inflammation and pancreatic cancer: molecular and functional interactions between S100A8, S100A9, NT-S100A8 and TGFβ1.
Basso D, Bozzato D, Padoan A, Moz S, Zambon CF, Fogar P, Greco E, Scorzeto M, Simonato F, Navaglia F, Fassan M, Pelloso M, Dupont S, Pedrazzoli S, Fassina A, Plebani M
Cell communication and signaling : CCS 2014 Mar 26;12:20
Cell communication and signaling : CCS 2014 Mar 26;12:20
Receptor for advanced glycation end products regulates adipocyte hypertrophy and insulin sensitivity in mice: involvement of Toll-like receptor 2.
Monden M, Koyama H, Otsuka Y, Morioka T, Mori K, Shoji T, Mima Y, Motoyama K, Fukumoto S, Shioi A, Emoto M, Yamamoto Y, Yamamoto H, Nishizawa Y, Kurajoh M, Yamamoto T, Inaba M
Diabetes 2013 Feb;62(2):478-89
Diabetes 2013 Feb;62(2):478-89
In vitro effects of atorvastatin on lipopolysaccharide-induced gene expression in endometriotic stromal cells.
Sharma I, Dhawan V, Mahajan N, Saha SC, Dhaliwal LK
Fertility and sterility 2010 Oct;94(5):1639-46.e1
Fertility and sterility 2010 Oct;94(5):1639-46.e1
Low anticoagulant heparin targets multiple sites of inflammation, suppresses heparin-induced thrombocytopenia, and inhibits interaction of RAGE with its ligands.
Rao NV, Argyle B, Xu X, Reynolds PR, Walenga JM, Prechel M, Prestwich GD, MacArthur RB, Walters BB, Hoidal JR, Kennedy TP
American journal of physiology. Cell physiology 2010 Jul;299(1):C97-110
American journal of physiology. Cell physiology 2010 Jul;299(1):C97-110
Differentiated human alveolar epithelial cells and reversibility of their phenotype in vitro.
Wang J, Edeen K, Manzer R, Chang Y, Wang S, Chen X, Funk CJ, Cosgrove GP, Fang X, Mason RJ
American journal of respiratory cell and molecular biology 2007 Jun;36(6):661-8
American journal of respiratory cell and molecular biology 2007 Jun;36(6):661-8
Receptor for advanced glycation endproducts mediates neutrophil migration across intestinal epithelium.
Zen K, Chen CX, Chen YT, Wilton R, Liu Y
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 15;178(4):2483-90
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 15;178(4):2483-90
Receptor for advanced glycation endproducts mediates neutrophil migration across intestinal epithelium.
Zen K, Chen CX, Chen YT, Wilton R, Liu Y
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 15;178(4):2483-90
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 15;178(4):2483-90
Distribution of the receptor for advanced glycation end products in the human male reproductive tract: prevalence in men with diabetes mellitus.
Mallidis C, Agbaje I, Rogers D, Glenn J, McCullough S, Atkinson AB, Steger K, Stitt A, McClure N
Human reproduction (Oxford, England) 2007 Aug;22(8):2169-77
Human reproduction (Oxford, England) 2007 Aug;22(8):2169-77
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human RAGE by Western Blot. Western blot shows lysates of human lung tissue and HEK001 human epidermal keratinocyte cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1145) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for RAGE at approximately 43 kDa under non-reducing (NR) conditions and 50 kDa under reducing (R) conditions (as indicated). This experiment was conducted using Immunoblot Buffer Group 5.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat RAGE by Western Blot. Western blot shows lysates of human, mouse, and rat lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1145) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for RAGE at approximately 45-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- RAGE in Human Alzheimer's Disease Brain. RAGE was detected in immersion fixed paraffin-embedded sections of human Alzheimer's disease brain (cerebellum) using 15 µg/mL Goat Anti-Human/Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1145) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- RAGE in Human Lung. RAGE was detected in immersion fixed paraffin-embedded sections of human lung using Goat Anti-Human/Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1145) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
