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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MAB1145 - Provider product page
- Provider
- R&D Systems
- Product name
- Human RAGE Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human RAGE in direct ELISAs and Western blots. In Western blots, this antibody shows no cross-reactivity with recombinant rat (rr) RAGE or rmRAGE.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q15109- Isotype
- IgG
- Antibody clone number
- 176907
- Vial size
- 500 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Post-apoptotic tumors are more palatable to dendritic cells and enhance their antigen cross-presentation activity.
IL-1alpha and IL-1beta are endogenous mediators linking cell injury to the adaptive alloimmune response.
IL-1alpha and IL-1beta are endogenous mediators linking cell injury to the adaptive alloimmune response.
RAGE-NF-kappaB pathway activation in response to oxidative stress in facioscapulohumeral muscular dystrophy.
Brusa D, Garetto S, Chiorino G, Scatolini M, Migliore E, Camussi G, Matera L
Vaccine 2008 Nov 25;26(50):6422-32
Vaccine 2008 Nov 25;26(50):6422-32
IL-1alpha and IL-1beta are endogenous mediators linking cell injury to the adaptive alloimmune response.
Rao DA, Tracey KJ, Pober JS
Journal of immunology (Baltimore, Md. : 1950) 2007 Nov 15;179(10):6536-46
Journal of immunology (Baltimore, Md. : 1950) 2007 Nov 15;179(10):6536-46
IL-1alpha and IL-1beta are endogenous mediators linking cell injury to the adaptive alloimmune response.
Rao DA, Tracey KJ, Pober JS
Journal of immunology (Baltimore, Md. : 1950) 2007 Nov 15;179(10):6536-46
Journal of immunology (Baltimore, Md. : 1950) 2007 Nov 15;179(10):6536-46
RAGE-NF-kappaB pathway activation in response to oxidative stress in facioscapulohumeral muscular dystrophy.
Macaione V, Aguennouz M, Rodolico C, Mazzeo A, Patti A, Cannistraci E, Colantone L, Di Giorgio RM, De Luca G, Vita G
Acta neurologica Scandinavica 2007 Feb;115(2):115-21
Acta neurologica Scandinavica 2007 Feb;115(2):115-21
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human RAGE by Western Blot. Western blot shows lysates of human lung tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human RAGE Monoclonal Antibody (Catalog # MAB1145) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for RAGE at approximately 43 kDa under non-reducing (NR) conditions and 50 kDa under reducing (R) conditions (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- RAGE in Human Liver Cancer Tissue. RAGE was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Mouse Anti-Human RAGE Monoclonal Antibody (Catalog # MAB1145) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
