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LearnPA5-21698
antibody from Invitrogen Antibodies
Targeting: BUD23
MERM1, MGC19709, MGC2022, MGC5140, PP3381, WBMT, WBSCR22
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-21698 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- WBSCR22 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, HCT116, A375M. Predicted reactivity: Rhesus Monkey (99%), Bovine (83%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.42 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of WBSCR22 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: WBSCR22 Polyclonal Antibody (Product # PA5-21698) diluted at 1:200. Red: FIS1, a mitochondria marker. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western BlotSCR22 Polyclonal Antibody immunoprecipitates Western BlotSCR22 protein in IP experiments. IP Sample: 1,000 µg HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2.5 µg of preimmune rabbit IgG C. Immunoprecipitation of Western BlotSCR22 protein by 2.5 µg of Western BlotSCR22 Polyclonal Antibody (Product # PA5-21698) 12% SDS-PAGE The immunoprecipitated Western BlotSCR22 protein was detected by Western BlotSCR22 Polyclonal Antibody (Product # PA5-21698) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using Western BlotSCR22 (Product # PA5-21698) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western BlotSCR22 Polyclonal Antibody detects Western BlotSCR22 protein at cytoplasm on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Western BlotSCR22 Polyclonal Antibody (Product # PA5-21698) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western BlotSCR22 Polyclonal Antibody immunoprecipitates Western BlotSCR22 protein in IP experiments. IP Sample: 1,000 µg HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2.5 µg of preimmune rabbit IgG C. Immunoprecipitation of Western BlotSCR22 protein by 2.5 µg of Western BlotSCR22 Polyclonal Antibody (Product # PA5-21698) 12% SDS-PAGE The immunoprecipitated Western BlotSCR22 protein was detected by Western BlotSCR22 Polyclonal Antibody (Product # PA5-21698) diluted at 1:1,000.
