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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [5]
- Other assay [2]
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- Product number
- PA5-52514 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-GGT5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: GTSHVSVLGE DGSAVAATST INTPFGAMVY SPRTGIILNN ELLDLCERCP RGSGTTPSPV SGDRVGGAPG RCWPPVPGER SPSSMVPSIL INKAQGSKLV IGGAGGELII SAVAQAIMSK LWLGFDLRAA IAAPILHVNS KG Highest antigen sequence identity to the following orthologs: Mouse - 77%, Rat - 30%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.05 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references S-Geranylgeranyl-L-glutathione is a ligand for human B cell-confinement receptor P2RY8.
Lu E, Wolfreys FD, Muppidi JR, Xu Y, Cyster JG
Nature 2019 Mar;567(7747):244-248
Nature 2019 Mar;567(7747):244-248
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of GGT5 in human cell line U-2 OS using a GGT5 Polyclonal Antibody (Product # PA5-52514) shows localization to nucleoli fibrillar center.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GGT5 in human adrenal gland using a GGT5 Polyclonal Antibody (Product # PA5-52514) shows strong positivity in extracellular matrix.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GGT5 in human tonsil using GGT5 Polyclonal Antibody (Product # PA5-52514) shows strong membranous positivity in follicular dendritic cells in germinal center.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GGT5 in human endometrium using GGT5 Polyclonal Antibody (Product # PA5-52514) shows moderate positivity in endothelial cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GGT5 in human kidney using GGT5 Polyclonal Antibody (Product # PA5-52514) shows moderate to strong membranous positivity in connective tissue cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GGT5 in human cerebellum using GGT5 Polyclonal Antibody (Product # PA5-52514) shows strong membranous positivity in endothelial cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Extended Data Figure 6. Expression of Ggt5 by human tonsil follicular dendritic cells and fragmentation pattern of S-geranylgeranyl-L-Cys-Gly. (a) P2RY8 ligand bioassay on supernatants from HEK293T cells transfected with the indicated enzymes (n=4 biological replicates). (b) Positive ion mode MS/MS spectra of the m/z 451.3 metabolite from extracts of the type in Figure 4d , corresponding to S-geranylgeranyl-L-Cys-Gly. (c) Immunohistochemistry for GGT5 or CR2 (brown), in serial sections of human tonsil, counterstained with hematoxylin (blue). Scale bars, 200 mum. (d) Immunofluorescence for GGT5 (green), CR2 (red), and DAPI (blue) in tonsil sections. Serial sections were stained for P2RY8 (green) and DAPI (blue) to visualize the difference between FDC-extensions and GC B-cell membranes. The indicated regions in the upper panels (scale bars, 100 mum) are enlarged in the lower panels (scale bars, 25 mum). (e) qPCR for GGT5 in the indicated tissues and cells from human tonsil, relative to GAPDH . Points within each category represent individual tonsils (whole tonsil=4, tonsil stroma=4, bulk lymphocytes=4, FoB=2, GC B=3, CXCR5 - CD4 + T=3, CXCR5 int CD4 + T=2, Tfh=3). Data are representative of or pooled from 2 (a,b) independent experiments and data in (c, d) are representative of 4 tonsil specimens. Graphs depict mean with s.d.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4. Ggt5 metabolizes Ggg and regulates P2RY8 function in vivo. (a, b) Flow cytometry plots (a) and summary data (b) of P2RY8 ligand bioassay of supernatants from the indicated cells overexpressing Ggt5 or empty vector (EV), cultured for 18 hr with Ggg or vehicle (HEK293T n=6, Hepa1-6 n=4 biological replicates, one-way ANOVA with Bonferroni's multiple comparisons test). Graphs depict mean with s.d. (c) Diagram of Ggg conversion into S-geranylgeranyl-L-Cys-Gly (GG-Cys-Gly). (d) Positive precursor ion scan for m/z 179 to identify ions producing [Cys-Gly] + fragments, from purified supernatants of HEK293T cells overexpressing EV or Ggt5 and incubated with Ggg. (e) LC-MS/MS MRM for Ggg and GG-Cys-Gly in a mixture of 100 nM Ggg and GG-Cys-Gly, or in C18 SPE concentrates of mouse spleen. (f) Immunohistochemistry for GGT5 or CR2 (brown) in serial sections of tonsil counterstained with hematoxylin (blue). (g) RNAscope for Ggt5 mRNA (red) in mouse lymph node GCs and primary follicles, counterstained with IgD (brown). Serial sections were stained for Cr1 (blue) and IgD (brown). (h) Immunofluorescence for P2RY8-overexpressing B cells (GFP, green) co-transferred with EV or Ggt5-overexpressing B cells, in unimmunized mice without GCs (top), and in GCs (Cr1, red) of immunized mice (bottom), relative to endogenous B cells (IgD, blue). Data are representative of or pooled from 3 (a,b,e) or 2 (d,h) experiments, 3 (h) or 5 (f,g) biological replicates. Scale bars, 100 mum.
