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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-22208 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Apoptosis-Enhancing Nuclease Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: HeLa, HepG2, Molt-4.
- Concentration
- 0.9 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Apoptosis-Enhancing Nuclease Polyclonal Antibody (Product # PA5-22208). Sample (30 µg of whole cell lysate). Lane A: Hela. 10% SDS PAGE. Apoptosis-Enhancing Nuclease Polyclonal Antibody (Product # PA5-22208) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Apoptosis-Enhancing Nuclease Polyclonal Antibody detects Apoptosis-enhancing nuclease protein at nucleus by immunofluorescent analysis. Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Apoptosis-enhancing nuclease protein stained by Apoptosis-Enhancing Nuclease Polyclonal Antibody (Product # PA5-22208) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using Apoptosis-enhancing nuclease (Product # PA5-22208) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
