18727-1-AP

antibody from Proteintech Group

Targeting: DNA2 DNA2L, KIAA0083

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

18727-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#18727-1-AP, RRID:AB_10637998

Product name

DNA2 antibody

Antibody type

Polyclonal

Description

KD/KO validated DNA2 antibody (Cat. #18727-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Polθ activity modulates sensitivity to standard therapies in DNMT3A-deficient leukemia.

Le BV, Vekariya U, Toma MM, Nieborowska-Skorska M, Caron MC, Gozdecka M, Haydar Z, Walsh M, Ghosh J, Vaughan-Williams E, Podszywalow-Bartnicka P, Kukuyan AM, Ziolkowska S, Atkins J, Hadzijusufovic E, Chandramouly G, Nejati R, Piwocka K, Pomerantz R, Vassiliou GS, Huntly BJP, Valent P, Wasik M, Bellacosa A, Masson JY, Gupta GP, Challen GA, Skorski T
Cell reports. Medicine 2026 Mar 17;7(3):102687

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Loss of CTLH component MAEA impairs DNA repair and replication and leads to developmental delay.

Hough SH, Jhujh SS, Awwad SW, Lewis OE, Lam S, Thomas JC, Mosler T, Bader A, Bartik L, McKee S, Amudhavalli S, Colin E, Damseh N, Clement E, Cacheiro P, Majumdar A, Smedley D, Fluss J, Giannini R, Thiffault I, Zagnoli Vieira G, Belotserkovskaya R, Smerdon SJ, Beli P, Galanty Y, Carnie CJ, Stewart GS, Jackson SP
EMBO molecular medicine 2026 Feb;18(2):492-513

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Phase separation of ERCC6L2-CtIP regulates the extent of DNA end resection.

Yin Y, Lin J, Cai X, Nie R, Lin J, Li S, Xiang Z, Ling Y, Zhang Y, Zhou J, Chen J, Lin W, Duan J, Zheng X, Xie D, Cai M
Nature cell biology 2025 Oct;27(10):1771-1784

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Isoform-specific function of NSD3 in DNA replication stress confers resistance to PARP inhibitors in prostate cancer.

Zhu S, Gao H, Jiang D, Guo G, Hou J, Han Y, Zhang C, Hu X, Indulkar S, Kloeber JA, Hu Q, Jiang Y, Zeng X, Ouyang Y, Lu J, Yin P, Luo K, Huang J, Wu Z, Chen B, Xiao H, Dragojevic S, Qin H, Zhou X, Jin J, Tu X, Wang L, Lou Z
Molecular cell 2025 Jul 17;85(14):2673-2687.e8

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DNA2 knockout aggravates cerebral ischemia/reperfusion injury by reducing postsynaptic Homer1a.

Ma T, Li YM, Ren PY, Wang SQ, Liu XL, Lv WB, Hou WG, Zuo WQ, Lin WQ, Sima J, Geng AQ
Zoological research 2025 Jan 18;46(1):87-102

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Dynamic control of RNA-DNA hybrid formation orchestrates DNA2 activation at stalled forks by RNAPII and DDX39A.

Song L, Xie H, Fan H, Zhang Y, Cheng Z, Chen J, Guo Y, Zhang S, Zhou X, Li Z, Liao H, Han J, Huang J, Zhou J, Fang D, Liu T
Molecular cell 2025 Feb 6;85(3):506-522.e7

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DDX39A resolves replication fork-associated RNA-DNA hybrids to balance fork protection and cleavage for genomic stability maintenance.

Xu Z, Nie C, Liao J, Ma Y, Zhou XA, Li X, Li S, Lin H, Luo Y, Cheng K, Mao Z, Zhang L, Pan Y, Chen Y, Wang W, Wang J
Molecular cell 2025 Feb 6;85(3):490-505.e11

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DNA polymerase theta-mediated DNA repair is a functional dependency and therapeutic vulnerability in DNMT3A deficient leukemia cells.

Le BV, Vekariya U, Toma MM, Nieborowska-Skorska M, Caron MC, Gozdecka M, Haydar Z, Walsh M, Ghosh J, Vaughan-Williams E, Podszywalow-Bartnicka P, Kukuyan AM, Ziolkowska S, Hadzijusufovic E, Chandramouly G, Piwocka K, Pomerantz R, Vassiliou GS, Huntly BJ, Valent P, Bellacosa A, Masson JY, Gupta GP, Challen GA, Skorski T
bioRxiv : the preprint server for biology 2024 Sep 19;

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Identification of Brain Cell Type-Specific Therapeutic Targets for Glioma From Genetics.

Gui J, Chen J, Wan K, Liu Y, Huang K, Zhu X
CNS neuroscience & therapeutics 2024 Dec;30(12):e70185

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The germline factor DDX4 contributes to the chemoresistance of small cell lung cancer cells.

Noyes C, Kitajima S, Li F, Suita Y, Miriyala S, Isaac S, Ahsan N, Knelson E, Vajdi A, Tani T, Thai TC, Xu D, Murai J, Tapinos N, Takahashi C, Barbie DA, Yajima M
Communications biology 2023 Jan 18;6(1):65

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Human HELQ regulates DNA end resection at DNA double-strand breaks and stalled replication forks.

Zhao Y, Hou K, Li Y, Hao S, Liu Y, Na Y, Li C, Cui J, Xu X, Wu X, Wang H
Nucleic acids research 2023 Dec 11;51(22):12207-12223

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Stalled replication fork protection limits cGAS-STING and P-body-dependent innate immune signalling.

Emam A, Wu X, Xu S, Wang L, Liu S, Wang B
Nature cell biology 2022 Jul;24(7):1154-1164

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Increased Resection at DSBs in G(2)-Phase Is a Unique Phenotype Associated with DNA-PKcs Defects That Is Not Shared by Other Factors of c-NHEJ.

Xiao H, Li F, Mladenov E, Soni A, Mladenova V, Pan B, Dueva R, Stuschke M, Timmermann B, Iliakis G
Cells 2022 Jul 2;11(13)

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Increased Expression of DNA2 Was Linked to Poor Prognosis in Breast Cancer.

Han Y, Zhang Z, Wang Z, Sun S
Disease markers 2021;2021:8860728

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Characteristic Analysis of Featured Genes Associated With Stemness Indices in Colorectal Cancer.

Lu Y, Zhou X, Liu Z, Wang W, Li F, Fu W
Frontiers in molecular biosciences 2020;7:563922

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Abro1 maintains genome stability and limits replication stress by protecting replication fork stability.

Xu S, Wu X, Wu L, Castillo A, Liu J, Atkinson E, Paul A, Su D, Schlacher K, Komatsu Y, You MJ, Wang B
Genes & development 2017 Jul 15;31(14):1469-1482

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The autophagy GABARAPL1 gene is epigenetically regulated in breast cancer models.

Hervouet E, Claude-Taupin A, Gauthier T, Perez V, Fraichard A, Adami P, Despouy G, Monnien F, Algros MP, Jouvenot M, Delage-Mourroux R, Boyer-Guittaut M
BMC cancer 2015 Oct 17;15:729

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The checkpoint clamp protein Rad9 facilitates DNA-end resection and prevents alternative non-homologous end joining.

Tsai FL, Kai M
Cell cycle (Georgetown, Tex.) 2014;13(21):3460-4

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The DNA2 antibody from Proteintech is a rabbit polyclonal antibody to a peptide of human DNA2. This antibody recognizes human antigen. The DNA2 antibody has been validated for the following applications: ELISA, IHC, IF analysis.

Appendix

18727-1-AP.pdf

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Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human cervical cancer using 18727-1-AP(DNA2 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human cervical cancer using 18727-1-AP(DNA2 antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue