FNab02433

antibody from FineTest Biotech
Targeting: DNA2 DNA2L, KIAA0083
Provider product page for FNab02433
Western blot
ELISA
Immunocytochemistry
Immunohistochemistry

Antibody data

Product number
FNab02433 - Provider product page
Provider
FineTest Biotech
Product name
DNA2 antibody
Antibody type
Polyclonal
Antigen
Recombinant protein fragment
Description
Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex(RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break(DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA(ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function.
Reactivity
Human, Mouse
Host
Rabbit
Conjugate
Unconjugated
Antigen sequence
DNA replication helicase 2 homolog
Epitope
IgG
Isotype
IgG
Vial size
100¦Ìg
Concentration
Immunogen affinity purified
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20¡æ for 12 months(Avoid repeated freeze / thaw cycles.)
Handling
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20¡æ for 12 months(Avoid repeated freeze / thaw cycles.)
DNA2 protein structure - FNab02433 shown in red.