PA5-64163

antibody from Invitrogen Antibodies

Targeting: GALNT7 GALNAC-T7

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-64163

Provider

Invitrogen Antibodies

Product name

GALNT7 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Immunogen sequence: GDSQKDIMQR QYLTFKPQTF TYHDPVLRPG ILGNFEPKEP EPPGVVGGPG EKAKPLVLGP EFKRAI Highest antigen sequence identity to the following orthologs: Mouse - 88%, Rat - 89%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Concentration

0.2 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

miR-30b-5p inhibits proliferation, invasion, and migration of papillary thyroid cancer by targeting GALNT7 via the EGFR/PI3K/AKT pathway.

Wang Y, Wang C, Fu Z, Zhang S, Chen J
Cancer cell international 2021 Nov 24;21(1):618

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of GALNT7 in human cell line HaCaT using a GALNT7 Polyclonal Antibody (Product # PA5-64163) shows localization to the Golgi apparatus.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of GALNT7 in human cell line HaCaT using a GALNT7 Polyclonal Antibody (Product # PA5-64163) shows localization to the Golgi apparatus.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of GALNT7 in human stomach using GALNT7 Polyclonal Antibody (Product # PA5-64163) shows high expression.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of GALNT7 in human colon, kidney, liver and stomach using GALNT7 Polyclonal Antibody (Product # PA5-64163) (A) shows similar protein distribution across tissues to an independent GALNT7 Polyclonal Antibody (B).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of GALNT7 in human liver using GALNT7 Polyclonal Antibody (Product # PA5-64163) shows low expression as expected.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of GALNT7 in human kidney using GALNT7 Polyclonal Antibody (Product # PA5-64163).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of GALNT7 in human colon using GALNT7 Polyclonal Antibody (Product # PA5-64163).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 7 GALNT7 promotes cell proliferation and invasion by activating EGFR/PI3K/AKT pathway. A , B GO and KEGG analysis was performed using the GSEA software. C , D Western blot analysis was used to detect the protein expression levels of EGFR, PI3K, p-PI3K, AKT, p-AKT and GALNT7 after transfection respectively. E Quantification of C . F Quantification of D . Data are presented as the mean +- SD (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001)