HPA001589

antibody from Atlas Antibodies

Targeting: SYTL4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA001589

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA001589, RRID:AB_1080174

Product name

Anti-SYTL4

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

RLKNELLEIKRKGAKRGSQHYSDRTCARCQESLGR
LSPKTNTCRGCNHLVCRDCRIQESNGTWRCKVCAK
EIELKKATGDWFYDQKVNRFAYRTGSEIIRMSLRH
KPAVSKRETVGQSLLHQTQMGDIWPGRKIIQERQK
EPSVLFEVP


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1, using Anti-SYTL4 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Negative control (vector only transfected HEK293T lysate)Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY409049)

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, cytosol & microtubule organizing center.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity in exocrine glandular cells.

Sample type

HUMAN