PAB20071

antibody from Abnova Corporation

Targeting: SYTL4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20071

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20071, RRID:AB_10968562

Product name

SYTL4 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant SYTL4.

Antigen sequence

RLKNELLEIKRKGAKRGSQHYSDRTCARCQESLGR
LSPKTNTCRGCNHLVCRDCRIQESNGTWRCKVCAK
EIELKKATGDWFYDQKVNRFAYRTGSEIIRMSLRH
KPAVSKRETVGQSLLHQTQMGDIWPGRKIIQERQK
EPSVLFEVP


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with SYTL4 polyclonal antibody (Cat # PAB20071) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with SYTL4 polyclonal antibody (Cat # PAB20071) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli, cytoplasm, centrosome.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas with SYTL4 polyclonal antibody (Cat # PAB20071) shows moderate cytoplasmic positivity in exocrine glandular cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)