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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Flow cytometry [2]
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Validation data
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- Product number
- PA5-25305 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IFT172 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with rat based on sequence homology.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Murine germ cell-specific disruption of Ift172 causes defects in spermiogenesis and male fertility.
Zhang S, Liu Y, Huang Q, Yuan S, Liu H, Shi L, Yap YT, Li W, Zhen J, Zhang L, Hess RA, Zhang Z
Reproduction (Cambridge, England) 2020 Apr;159(4):409-421
Reproduction (Cambridge, England) 2020 Apr;159(4):409-421
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IFT172 in mouse lung tissue lysates. Samples were incubated with IFT172 polyclonal antibody (Product # PA5-25305). Lysates: 35 µg/lane. IFT172 protein (arrow).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of 293 cells using an IFT172 polyclonal antibody (Product # PA5-25305) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of IFT172 in 293 cells (right histogram). Samples were incubated with IFT172 polyclonal antibody (Product # PA5-25305) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (left histogram).
