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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- PA5-53050 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PARD6B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: NYHKAVSTAN PLLRIFIQKK EEADYSAFGT DTLIKKKNVL TNVLRPDNHR KKPHIVISMP QDFRPVSSII DVDILPETHR RV
- Concentration
- 0.10 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of PARD6B was achieved by transfecting T-47D with PPP1R9B specific siRNAs (Silencer® select Product # s39171, s39172). Western blot analysis (Fig. a) was performed using whole cell extracts from the PARD6B knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with PARD6B Polyclonal Antibody (Product # PA5-53050, 1ug/ml) and chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Densitometric analysis of this western blot is shown in histogram (Fig. b) Corresponding to top band and (Fig. c) corresponding to lower band. Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to PARD6B.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-PARD6B Polyclonal clonal Antibody (Product # PA5-53050) and a 50kDa band corresponding to PARD6B was observed across cell lines and tissues tested. Whole cell Extracts (30 µg lysate) of MCF-7 (Lane 1), MDA-MB-231 (Lane 2), T-47D (Lane 3), A-549 (Lane 4), Mouse Skeletal Muscle (Lane 5) and Mouse Spleen (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1ug/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of PARD6B in human cell line U-2 OS shows positivity in cytoplasm. Samples were probed using a PARD6B Polyclonal Antibody (Product # PA5-53050).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PARD6B in human lung using PARD6B Polyclonal Antibody (Product # PA5-53050) shows moderate cytoplasmic positivity in macrophages.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PARD6B in human kidney using PARD6B Polyclonal Antibody (Product # PA5-53050) shows weak to moderate cytoplasmic positivity in cells in tubules.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PARD6B in human placenta using PARD6B Polyclonal Antibody (Product # PA5-53050) shows weak cytoplasmic positivity in trophoblastic cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PARD6B in human pancreas using PARD6B Polyclonal Antibody (Product # PA5-53050) shows very weak positivity in exocrine glandular cells.
