Explore
Validate
Learn
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-103220 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NOX1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Antibody detects endogenous levels of total NOX1.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Setanaxib (GKT137831) Ameliorates Doxorubicin-Induced Cardiotoxicity by Inhibiting the NOX1/NOX4/Reactive Oxygen Species/MAPK Pathway.
Zheng H, Xu N, Zhang Z, Wang F, Xiao J, Ji X
Frontiers in pharmacology 2022;13:823975
Frontiers in pharmacology 2022;13:823975
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NOX1 in K562 cell lysate. Samples were incubated with NOX1 polyclonal antibody (Product # PA5-103220).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NOX1 in mouse kidney and rat brain. Samples were incubated with NOX1 polyclonal antibody (Product # PA5-103220).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NOX1 in 293 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with NOX1 polyclonal antibody (Product # PA5-103220) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NOX1 in 293 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with NOX1 polyclonal antibody (Product # PA5-103220) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NOX1 in HepG2 cell lysate. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with NOX1 polyclonal antibody (Product # PA5-103220) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 1 The protein levels of NOX1 and NOX4 were increased by DOX both in vivo and in vitro . (A) Representative western blot analysis of NOX1 and NOX4 in myocardial tissue. (B,C) Quantification of NOX1 and NOX4 expression relative to the beta-actin level ( n = 6 per group). (D,E) Representative images of NOX1 and NOX4 expression in myocardial tissue detected by the immunofluorescence. The nuclei were stained with DAPI (Scale bar = 50 um). (F) Representative western blot analysis of NOX1 and NOX4 of NRCMs treated with DOX of different concentrations for 24 h (G,H) Quantification of NOX1 and NOX4 expression relative to the beta-actin level ( n = 3). (I) Representative western blot analysis of NOX 1 and NOX 4 of NRCMs treated with DOX for different time. (J,K) Quantification of NOX1 and NOX4 expression relative to the beta-actin level ( n = 4-5). * p < 0.05, ** p < 0.01, *** p < 0.001, n. s., not significant. NOX, NADPH oxidase; DOX, doxorubicin; cTnI, Cardiac troponin I.
