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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- BAM853 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse GDF-5/BMP-14 Biotinylated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse GDF-5/BMP-14 in Western blots.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Biotin
- Antigen sequence
P43027- Isotype
- IgG
- Antibody clone number
- 90509
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- GDF-5 in Mouse Embryo. GDF-5 was detected in immersion fixed frozen sections of mouse embryo using Mouse GDF-5 Biotinylated Monoclonal Antibody (Catalog # BAM853) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
