H00003092-D01P

antibody from Abnova Corporation

Targeting: HIP1 ILWEQ

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003092-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003092-D01P, RRID:AB_1674147

Product name

HIP1 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human HIP1 protein.

Antigen sequence

MDRMASSMKQVPNPLPKVLSRRGVGAGLEAAERES
FERTQTVSINKAINTQEVAVKEKHARTCILGTHHE
KGAQTFWSVVNRLPLSSNAVLCWKFCHVFHKLLRD
GHPNVLKDSLRYRNELSDMSRMWGHLSEGYGQLCS
IYLKLLRTKMEYHTKNPRFPGNLQMSDRQLDEAGE
SDVNNFFQLTVEMFDYLECELNLFQTVFNSLDMSR
SVSVTAAGQCRLAPLIQVILDCSHLYDYTVKLLFK
LHSCLPADTLQGHRDRFMEQFTKLKDLFYRSSNLQ
YFKRLIQIPQLPENPPNFLRASALSEHISPVVVIP
AEASSPDSEPVLEKDDLMDMDASQQNLFDNKFDDI
FGSSFSSDPFNFNSQNGVNKDEKDHLIERLYREIS
GLKAQLENMKTESQRVVLQLKGHVSELEADLAEQQ
HLRQQAADDCEFLRAELDELRRQREDTEKAQRSLS
EIERKAQANEQRYSKLKEKYSELVQNHADLLRKNA
EVTKQVSMARQAQVDLEREKKELEDSLERISDQGQ
RKTQEQLEVLESLKQELATSQRELQVLQGSLETSA
QSEANWAAEFAELEKERDSLVSGAAHREEELSALR
KELQDTQLKLASTEESMCQLAKDQRKMLLVGSRKA
AEQVIQDALNQLEEPPLISCAGSADHLLSTVTSIS
SCIEQLEKSWSQYLACPEDISGLLHSITLLAHLTS
DAIAHGATTCLRAPPEPADSLTEACKQYGRETLAY
LASLEEEGSLENADSTAMRNCLSKIKAIGEELLPR
GLDIKQEELGDLVDKEMAATSAAIETATARIEEML
SKSRAGDTGVKLEVNERILGCCTSLMQAIQVLIVA
SKDLQREIVESGRGTASPKEFYAKNSRWTEGLISA
SKAVGWGATVMVDAADLVVQGRGKFEELMVCSHEI
AASTAQLVAASKVKADKDSPNLAQLQQASRGVNQA
TAGVVASTISGKSQIEETDNMDFSSMTLTQIKRQE
MDSQVRVLELENELQKERQKLGELRKKHYELAGVA
EGWEEGTEASPPTLQEVVTEKE


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of HIP1 expression in transfected 293T cell line (H00003092-T01) by HIP1 MaxPab polyclonal antibody.Lane 1: HIP1 transfected lysate(116.20 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between HIP1 and CLTC. HeLa cells were stained with anti-HIP1 rabbit purified polyclonal 1:1200 and anti-CLTC mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)