PA5-53068

antibody from Invitrogen Antibodies

Targeting: HIP1 ILWEQ

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-53068

Provider

Invitrogen Antibodies

Product name

HIP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: NFLRASALSE HISPVVVIPA EASSPDSEPV LEKDDLMDMD ASQQNLFDNK FDDIFGSSFS SDPFNFNSQN GVNKDEKDHL IERLYREISG LKAQLENMKT ESQRVVLQLK GHVSELEADL AEQQHLRQQA ADDCEFLRAE LDELRRQRED

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HIP1 in mouse cell line NIH-3T3 and rat cell line NBT-II using a HIP1 Polyclonal Antibody (Product # PA5-53068).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HIP1 in human cell lines A-549 and Caco-2 using a HIP1 Polyclonal Antibody (Product # PA5-53068). Corresponding HIP1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of HIP1 in human cell line U-251 MG shows positivity in vesicles. Samples were probed using a HIP1 Polyclonal Antibody (Product # PA5-53068).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of HIP1 in human kidney using HIP1 Polyclonal Antibody (Product # PA5-53068).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of HIP1 in human colon using HIP1 Polyclonal Antibody (Product # PA5-53068).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of HIP1 in human testis using HIP1 Polyclonal Antibody (Product # PA5-53068) shows high expression.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of HIP1 in human colon, kidney, liver and testis using HIP1 Polyclonal Antibody (Product # PA5-53068) (A) shows similar protein distribution across tissues to an independent HIP1 Polyclonal Antibody (B).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of HIP1 in human liver using HIP1 Polyclonal Antibody (Product # PA5-53068) shows low expression as expected.