PA5-109238

antibody from Invitrogen Antibodies

Targeting: HIP1 ILWEQ

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-109238

Provider

Invitrogen Antibodies

Product name

HIP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Positive Control: Mouse testis tissue lysate, Mouse spinal cord tissue lysate, A549, PC-3M, SH-SY5Y, human colon cancer tissue, mouse brain tissue, mouse fallopian tubes tissue.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Hip1 in Positive control: Lane 1: Mouse testis, Lane 2: Mouse spinal cord, Lane 3: SH-SY5Y. Samples were incubated with Hip1 polyclonal antibody (Product # PA5-109238), at a dilution of 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hip1 in SH-SY-5Y cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Hip1 polyclonal antibody (Product # PA5-109238), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hip1 in PC-3M cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Hip1 polyclonal antibody (Product # PA5-109238), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hip1 in A549 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Hip1 polyclonal antibody (Product # PA5-109238), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Hip1 in paraffin-embedded mouse fallopian tubes tissue. Samples were incubated with Hip1 polyclonal antibody (Product # PA5-109238), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Hip1 in paraffin-embedded mouse brain tissue. Samples were incubated with Hip1 polyclonal antibody (Product # PA5-109238), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Hip1 in paraffin-embedded human colon cancer tissue. Samples were incubated with Hip1 polyclonal antibody (Product # PA5-109238), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of Hip1 in SH-SY5Y cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with Hip1 polyclonal antibody (Product # PA5-109238) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.