Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 48-9952-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-HLA-DR Monoclonal Antibody (L243), eFluor 450, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The L243 mAb reacts with the human major histocompatibility complex (MHC) class II, HLA-DR. HLA-DR is expressed on the surface of human antigen presenting cells (APC) including B cells, monocytes, macrophages, DCs, and activated T cells. HLA-DR is a heterodimeric transmembrane protein composed of alpha and beta subunits and plays an important role in the presentation of peptides to CD4^+ T lymphocytes. The L243 antibody recognizes a different epitope than the LN3 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes. Applications Reported: This L243 antibody has been reported for use in flow cytometric analysis. . Applications Tested: This L243 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- L243
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Accumulation of T-helper 22 cells, interleukin-22 and myeloid-derived suppressor cells promotes gastric cancer progression in elderly patients.
Human umbilical cord mesenchymal stem cells improve the reserve function of perimenopausal ovary via a paracrine mechanism.
ICOS+ Foxp3+ TILs in gastric cancer are prognostic markers and effector regulatory T cells associated with Helicobacter pylori.
Antithymocyte Globulin at Clinically Relevant Concentrations Kills Leukemic Blasts.
Remarkably low affinity of CD4/peptide-major histocompatibility complex class II protein interactions.
A humanized bone marrow ossicle xenotransplantation model enables improved engraftment of healthy and leukemic human hematopoietic cells.
Plasmacytoid dendritic cells are scarcely represented in the human gut mucosa and are not recruited to the celiac lesion.
Rapid accumulation of CD14+CD11c+ dendritic cells in gut mucosa of celiac disease after in vivo gluten challenge.
A matrix approach to human class II histocompatibility antigens: reactions of four monoclonal antibodies with the products of nine haplotypes.
Studies of a human T lymphocyte antigen recognized by a monoclonal antibody.
Chen X, Wang Y, Wang J, Wen J, Jia X, Wang X, Zhang H
Oncology letters 2018 Jul;16(1):253-261
Oncology letters 2018 Jul;16(1):253-261
Human umbilical cord mesenchymal stem cells improve the reserve function of perimenopausal ovary via a paracrine mechanism.
Li J, Mao Q, He J, She H, Zhang Z, Yin C
Stem cell research & therapy 2017 Mar 9;8(1):55
Stem cell research & therapy 2017 Mar 9;8(1):55
ICOS+ Foxp3+ TILs in gastric cancer are prognostic markers and effector regulatory T cells associated with Helicobacter pylori.
Nagase H, Takeoka T, Urakawa S, Morimoto-Okazawa A, Kawashima A, Iwahori K, Takiguchi S, Nishikawa H, Sato E, Sakaguchi S, Mori M, Doki Y, Wada H
International journal of cancer 2017 Feb 1;140(3):686-695
International journal of cancer 2017 Feb 1;140(3):686-695
Antithymocyte Globulin at Clinically Relevant Concentrations Kills Leukemic Blasts.
Dabas R, Lee R, Servito MT, Dharmani-Khan P, Modi M, van Slyke T, Luider J, Durand C, Larratt L, Brandwein J, Morris D, Daly A, Khan FM, Storek J
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation 2016 May;22(5):815-24
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation 2016 May;22(5):815-24
Remarkably low affinity of CD4/peptide-major histocompatibility complex class II protein interactions.
Jönsson P, Southcombe JH, Santos AM, Huo J, Fernandes RA, McColl J, Lever M, Evans EJ, Hudson A, Chang VT, Hanke T, Godkin A, Dunne PD, Horrocks MH, Palayret M, Screaton GR, Petersen J, Rossjohn J, Fugger L, Dushek O, Xu XN, Davis SJ, Klenerman D
Proceedings of the National Academy of Sciences of the United States of America 2016 May 17;113(20):5682-7
Proceedings of the National Academy of Sciences of the United States of America 2016 May 17;113(20):5682-7
A humanized bone marrow ossicle xenotransplantation model enables improved engraftment of healthy and leukemic human hematopoietic cells.
Reinisch A, Thomas D, Corces MR, Zhang X, Gratzinger D, Hong WJ, Schallmoser K, Strunk D, Majeti R
Nature medicine 2016 Jul;22(7):812-21
Nature medicine 2016 Jul;22(7):812-21
Plasmacytoid dendritic cells are scarcely represented in the human gut mucosa and are not recruited to the celiac lesion.
Ráki M, Beitnes AC, Lundin KE, Jahnsen J, Jahnsen FL, Sollid LM
Mucosal immunology 2013 Sep;6(5):985-92
Mucosal immunology 2013 Sep;6(5):985-92
Rapid accumulation of CD14+CD11c+ dendritic cells in gut mucosa of celiac disease after in vivo gluten challenge.
Beitnes AC, Ráki M, Brottveit M, Lundin KE, Jahnsen FL, Sollid LM
PloS one 2012;7(3):e33556
PloS one 2012;7(3):e33556
A matrix approach to human class II histocompatibility antigens: reactions of four monoclonal antibodies with the products of nine haplotypes.
Brodsky FM
Immunogenetics 1984;19(3):179-94
Immunogenetics 1984;19(3):179-94
Studies of a human T lymphocyte antigen recognized by a monoclonal antibody.
Engleman EG, Warnke R, Fox RI, Dilley J, Benike CJ, Levy R
Proceedings of the National Academy of Sciences of the United States of America 1981 Mar;78(3):1791-5
Proceedings of the National Academy of Sciences of the United States of America 1981 Mar;78(3):1791-5
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Mouse IgG2a kappa Isotype Control eFluor® 450 (Product # 48-4724-82) (blue histogram) or Anti-Human HLA-DR eFluor® 450 (purple histogram). Cells in the lymphocyte (left) or monocyte (right) gate were used for analysis.