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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [7]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-29772 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- VEGFC Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: TL1, mouse lung, Rat spleen, U87-MG conditioned medium.
- Concentration
- 0.3 mg/mL
Submitted references Intracellular calprotectin (S100A8/A9) controls epithelial differentiation and caspase-mediated cleavage of EGFR in head and neck squamous cell carcinoma.
Expression of angiogenic switch, cachexia and inflammation factors at the crossroad in undifferentiated thyroid carcinoma with BRAF(V600E).
Argyris PP, Slama Z, Malz C, Koutlas IG, Pakzad B, Patel K, Kademani D, Khammanivong A, Herzberg MC
Oral oncology 2019 Aug;95:1-10
Oral oncology 2019 Aug;95:1-10
Expression of angiogenic switch, cachexia and inflammation factors at the crossroad in undifferentiated thyroid carcinoma with BRAF(V600E).
Husain A, Hu N, Sadow PM, Nucera C
Cancer letters 2016 Oct 1;380(2):577-585
Cancer letters 2016 Oct 1;380(2):577-585
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of VEGFC in Rat tissue extracts (50 µg). Samples was separated by 10% SDS-PAGE and the membrane was probed with VEGFC Polyclonal antibody (Product # PA5-29772) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of VEGFC using 20 µg of A) TL1 whole cell lysate and B) TL1 knock down VEGF-C whole cell lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a VEGFC polyclonal antibody (Product # PA5-29772) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of VEGFC was performed by separating 30 µg of u87-MG whole cell extract and conditioned medium by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a VEGFC Polyclonal Antibody (Product # PA5-29772) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using VEGFC Polyclonal Antibody (Product # PA5-29772). Sample (20 µg of whole cell lysate). Lane A: TL1 whole cell lysate. Lane B: TL1 knock down VEGF-C whole cell lysate. 12% SDS PAGE. VEGFC Polyclonal Antibody (Product # PA5-29772) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- VEGFC Polyclonal Antibody detects VEGFC protein by western blot analysis. A. 50 µg mouse lung lysate/extract. 12% SDS-PAGE. VEGFC Polyclonal Antibody (Product # PA5-29772) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of VEGFC was performed by separating 30 µg of u87-MG whole cell extract and conditioned medium by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a VEGFC Polyclonal Antibody (Product # PA5-29772) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- VEGFC Polyclonal Antibody detects VEGFC protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with VEGFC Polyclonal Antibody (Product # PA5-29772) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human hepatoma, using VEGFC (Product # PA5-29772) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
