HPA012866

KIAA0513 antibody from Atlas Antibodies

Eligible for validation within the Antibodypedia Validation Initiative

Western blot

Enhanced validation

Immunohistochemistry

Enhanced validation

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Antibody Data

Product number

HPA012866

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA012866, RRID:AB_1234628

Product name

Anti-KIAA0513

Provider product page

Antibody type

Polyclonal

Description

Affinity purified using the PrEST antigen as affinity ligand

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

EWFARYVSAQRCNSKCVSEATFYRLVQSFAVVLFE
CHQMDDFGPAKNLMTMCFTYYHIGKPQLLPPESRE
KPAGSIDSYLKSANSWLAEKKDIAERLLKNTSART
ENVKGFFGGLETKLKGPLARRNEEDENKPQEKR


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and LY402367 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402367).

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in Purkinje cells and strong cytoplasmic positivity in cells in granular layer and molecular layer.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver shows low expression as expected.

Sample type

HUMAN

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human cerebral cortex and liver tissues using Anti-KIAA0513 antibody. Corresponding KIAA0513 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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