PA5-64393

antibody from Invitrogen Antibodies

Targeting: NCAPH2 384D8-2, CAP-H2, hCAP-H2

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-64393

Provider

Invitrogen Antibodies

Product name

NCAPH2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: EAENEFLSLD DFPDSRTNVD LKNDQTPSEV LIIPLLPMAL VAPDEMEKNN NPLYSRQGEV LASRKDFRMN TCVPHPRGAF MLEPEGMS Highest antigen sequence identity to the following orthologs: Mouse - 81%, Rat - 82%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.4 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Cell division requires RNA eviction from condensing chromosomes.

Sharp JA, Perea-Resa C, Wang W, Blower MD
The Journal of cell biology 2020 Nov 2;219(11)

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of NCAPH2 in human cell line HeLa shows positivity in cytokinetic bridge & nucleus but excluded from the nucleoli. Samples were probed using a NCAPH2 Polyclonal Antibody (Product # PA5-64393).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of NCAPH2 in human cell line MCF7 using a NCAPH2 Polyclonal Antibody (Product # PA5-64393) shows localization to nucleoplasm and cell junctions.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure S5. Cells expressing phosphomutant SAF-A show a transient defect in kinetochore-microtubule error correction but show normal localization of mitotic factors. (a-c) To test for correction of kinetochore-microtubule attachment errors, SAF-A wt -GFP and SAF-A S14A S26A -GFP cells were treated with monastrol and subsequently released into media containing MG132. For each time point in the experiment, 100-300 cells were scored. (a) Examples of each chromosome alignment category scored after monastrol washout. (b) Quantitation of bipolar spindle assembly following monastrol washout. Error bars are the SD values from two experiments. (c) Quantitation of chromosome alignment following monastrol washout. We observed a 2.5-fold increase in chromosome misalignment in SAF-A S14A S26A -GFP cells 30 min after monastrol release. Error bars are the SD from two experiments. (d) Localization of condensin I subunit NCAPD2 in SAF-A wt -GFP and SAF-A S14A S26A -GFP cells. 4x magnification was produced using Adobe Photoshop. (e) Localization of condensin II subunit NCAPH2 in SAF-A wt -GFP and SAF-A S14A S26A -GFP cells. 4x magnification was produced in Photoshop. (f) Violin plot depicting normalized Aurora-B centromere intensity in SAF-A wt -GFP and SAF-A S14A S26A -GFP cells. For replicate 1, SAF-A wt1 ( n = 415 kinetochores), SAF-A S14A S26A1 ( n = 704 kinetochores). In replicate 2, SAF-A wt2 ( n = 1,447 kinetochores), SAF-A S14A S26A2 ( n = 1,927 kinetochores). (g) Violin plot depicting